Application Data
TNF beta Recombinant Protein | LTA recombinant protein
TNF beta Protein, Human, Recombinant
Gene Names
LTA; LT; TNFB; TNFSF1
Purity
>97 % as determined by SDS-PAGE
Synonyms
TNF beta; N/A; TNF beta Protein, Human, Recombinant; Human TNF-beta/TNFSF1/Lymphotoxin alpha Protein; lymphotoxin alpha; LT Protein, Human; TNFB Protein, Human; TNFSF1 Protein, Human; LTA recombinant protein
Host
E Coli
Purity/Purification
>97 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 50mM Tris, pH8.0 Normally 5% trehalose, 5% mannitol and 0.01% Tween-80.
Sequence Positions
172aa
Sequence
Leu35-Leu205
Source
Human
Predicted N Terminal
Met
Molecular Mass
The recombinant human TNFβ comprises 172 amino acids and predicts a molecular mass of 18.8kDa as estimated in SDS-PAGE under reducing conditions.
Reconstitution
It is reconstituted that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
Concentration is measured by UV-Vis
Concentration is measured by UV-Vis
Protein Construction
|A DNA sequence encoding the mature form of human TNFβ (P01374) (Leu 35-Leu 205) was expressed and purified, with an initial Met at the N-terminus
Bio Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.01-0.1ng/mL.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70°C.
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles
Application Data
Application Data
Related Product Information for LTA recombinant protein
Lymphotoxin-alpha, also known as LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA TNFSF1, and TNFB, is a secreted protein that belongs to the tumor necrosis factor family. TNF-beta/TNFSF1/Lymphotoxin alpha is highly inducible, secreted, and exists as a homotrimeric molecule. It is a cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR, and TNFRSF14/HVEM. In its heterotrimeric form with LTB, TNF-beta/TNFSF1/Lymphotoxin alpha binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells. TNF-beta/TNFSF1/Lymphotoxin alpha forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. It mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. TNF-beta/TNFSF1/Lymphotoxin alpha is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in TNF-beta/TNFSF1/Lymphotoxin alpha are a cause of susceptibility psoriatic arthritis which is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy.
Product Categories/Family for LTA recombinant protein
References
Messer G, et al. (1991) Polymorphic structure of the tumor necrosis factor (TNF) locus: an NcoI polymorphism in the first intron of the human TNF-beta gene correlates with a variant amino acid in position 26 and a reduced level of TNF-beta production. J Exp Med. 173(1): 209-19.Banner DW, et al. (1993) Crystal structure of the soluble human 55 kd TNF receptor-human TNF beta complex: implications for TNF receptor activation. Cell. 73(3): 431-45.Picarella DE, et al. (1993) Transgenic tumor necrosis factor (TNF)-alpha production in pancreatic islets leads to insulitis, not diabetes. Distinct patterns of inflammation in TNF-alpha and TNF-beta transgenic mice. J Immunol. 150(9): 4136-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
lymphotoxin alpha
NCBI Official Synonym Full Names
lymphotoxin alpha
NCBI Official Symbol
LTA
NCBI Official Synonym Symbols
LT; TNFB; TNFSF1
NCBI Protein Information
lymphotoxin-alpha; LT-alpha; TNF-beta; TNF superfamily, member 1; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name
Lymphotoxin-alpha
UniProt Gene Name
LTA
UniProt Synonym Gene Names
TNFB; TNFSF1; LT-alpha
UniProt Entry Name
TNFB_HUMAN
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Product Notes
The LTA lta (Catalog #AAA257408) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 172aa. The amino acid sequence is listed below: Leu35-Leu2 05. It is sometimes possible for the material contained within the vial of "TNF beta, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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