Standard Curve (Sample)
Chicken Lymphotactin ELISA Kit | LTN elisa kit
Chicken Lymphotactin ELISA Kit
Gene Names
XCL1; LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
Reactivity
Chicken
Synonyms
Lymphotactin; N/A; Chicken Lymphotactin ELISA Kit; LTN elisa kit
Reactivity
Chicken
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
1.0 pg/mL.
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for LTN elisa kit
Intended Uses: This LTN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken LTN. This ELISA kit for research use only!
Principle of the Assay: LTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTN antibody and an LTN-HRP conjugate. The assay sample and buffer are incubated together with LTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTN concentration since LTN from samples and LTN-HRP conjugate compete for the anti-LTN antibody binding site. Since the number of sites is limited, as more sites are occupied by LTN from the sample, fewer sites are left to bind LTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTN concentration in each sample is interpolated from this standard curve.
Principle of the Assay: LTN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LTN antibody and an LTN-HRP conjugate. The assay sample and buffer are incubated together with LTN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LTN concentration since LTN from samples and LTN-HRP conjugate compete for the anti-LTN antibody binding site. Since the number of sites is limited, as more sites are occupied by LTN from the sample, fewer sites are left to bind LTN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LTN concentration in each sample is interpolated from this standard curve.
Product Categories/Family for LTN elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
12,517 Da
NCBI Official Full Name
lymphotactin
NCBI Official Synonym Full Names
chemokine (C motif) ligand 1
NCBI Official Symbol
XCL1
NCBI Official Synonym Symbols
LTN; ATAC; LPTN; SCM1; SCM-1; SCM1A; SCYC1; SCM-1a
NCBI Protein Information
lymphotactin; SCM-1-alpha; lymphotaxin; cytokine SCM-1; c motif chemokine 1; XC chemokine ligand 1; single cysteine motif 1a; small-inducible cytokine C1; small inducible cytokine subfamily C, member 1 (lymphotactin)
UniProt Protein Name
Lymphotactin
UniProt Gene Name
XCL1
UniProt Synonym Gene Names
LTN; SCYC1
UniProt Entry Name
XCL1_HUMAN
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Product Notes
The Chicken LTN xcl1 (Catalog #AAA93585) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA93585 ELISA Kit recognizes Chicken LTN. It is sometimes possible for the material contained within the vial of "Lymphotactin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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