T-Lymphocyte Surface Antigen Ly-9 Recombinant Protein | Ly9 recombinant protein

Recombinant Mouse T-Lymphocyte Surface Antigen Ly-9 Protein

Cat. Num. AAA174603

• Gene Names: Ly9; Ly-9; T100; CD229; Lgp100; SLAMF3; AI893573
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: T-Lymphocyte Surface Antigen Ly-9; N/A; Recombinant Mouse T-Lymphocyte Surface Antigen Ly-9 Protein; T-lymphocyte surface antigen Ly-9; Cell surface molecule Ly-9; Lymphocyte antigen 9; SLAM family member 3; SLAMF3; Signaling lymphocytic activation molecule 3; CD229; Ly9; Ly-9; AI893573; Lgp100; T100; Ly9 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, pH 7.4.
• Sequence Positions: Lys48-Phe454
• Sequence Length: 654

• Species: Mouse
• Endotoxin: <1.0 EU per mug as determined by LAL test.
• Fragment: Met 1-Arg 453
• Fusion Tag: C-6His
• Accession: Q4VBG4
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom.; Do not mix by vortex; It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized protein are stable for up to 12 months when stored at -20°C to -80°C.; Reconstituted protein solution can be stored at 4-7°C for 2-7 days.; Aliquots of reconstituted samples are stable at < -20°C for 3 months.

SDS-PAGE

Related Product Information for Ly9 recombinant protein

>Background: CD229(SLAMF3) is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family. Mature mouse CD229 consists of a 406 aa extracellular domain (ECD) with two Ig-like V-set and two Ig-like truncated C2-set domains, a 21 aa transmembrane segment, and a 180 aa cytoplasmic domain with two immunoreceptor tyrosinebased switch motifs ITSMs. Within the first two Ig-like domains that are common to all SLAM proteins, mouse CD229 shares 22%-36% aa sequence identity with mouse 2B4, BLAME, CD2F10,CD84, CRACC, NTBA, and SLAM. CD229 is expressed on T, B, and NK cells, thymocytes and monocytes. Homophilic binding between CD229 molecules is mediated by the N-terminal Ig-like domain. Human and mouse CD229 exhibit crossspecies binding. Antigen stimulation of lymphocytes induces CD229 clustering to sites of T cell-B cell contact. Antibody ligation of CD229 can inhibit T cell activation, but CD229 knockout mice show impaired T cell immune responses, suggesting a potential role for CD229 in T cell activation or costimulation.

NCBI and Uniprot Product Information

• NCBI GI #: 111955338
• NCBI GeneID: 17085
• NCBI Accession #: Q4VBG4
• NCBI GenBank Nucleotide #: NM_008534.3
• UniProt Accession #: Q01965; Q9ES29; Q9ES35; Q9ES36
• Molecular Weight: Molecular Mass: 47kDa; Actual Protein Molecular Mass: 65-75kDa
• NCBI Official Full Name: T-lymphocyte surface antigen Ly-9 isoform 1
• NCBI Official Synonym Full Names: lymphocyte antigen 9
• NCBI Official Symbol: Ly9
• NCBI Official Synonym Symbols: Ly-9; T100; CD229; Lgp100; SLAMF3; AI893573
• NCBI Protein Information: T-lymphocyte surface antigen Ly-9
• UniProt Protein Name: T-lymphocyte surface antigen Ly-9
• UniProt Gene Name: Ly9
• UniProt Synonym Gene Names: Ly-9; SLAMF3