Standard Curve (Sample)
Mouse MADCAM-1 ELISA Kit | MADCAM1 elisa kit
Mouse MADCAM-1 ELISA Kit
Gene Names
Madcam1; AV211525
Reactivity
Mouse
Applications
ELISA
Synonyms
MADCAM-1; N/A; Mouse MADCAM-1 ELISA Kit; Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 elisa kit
Reactivity
Mouse
Specificity
The Mouse MADCAM-1 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse MADCAM-1 proteins within the range of 125 pg/ml - 8000 pg/ml.
Sequence Length
405
Samples
Cell Culture Supernatants, Serum.
Sensitivity
10 pg/ml.
Applicable Applications for MADCAM1 elisa kit
ELISA (Sandwich ELISA)
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MADCAM1 elisa kit
Principle of the Assay||The Mouse MADCAM-1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse MADCAM-1 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Mouse MADCAM-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MADCAM-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse MADCAM-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MADCAM-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Background/Introduction: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4/beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
Background/Introduction: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4/beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
28,357 Da
NCBI Official Full Name
Mucosal addressin cell adhesion molecule 1
NCBI Official Synonym Full Names
mucosal vascular addressin cell adhesion molecule 1
NCBI Official Symbol
Madcam1
NCBI Official Synonym Symbols
AV211525
NCBI Protein Information
mucosal addressin cell adhesion molecule 1
UniProt Protein Name
Mucosal addressin cell adhesion molecule 1
UniProt Gene Name
Madcam1
UniProt Synonym Gene Names
MAdCAM-1; mMAdCAM-1
UniProt Entry Name
MADCA_MOUSE
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Product Notes
The Mouse MADCAM1 madcam1 (Catalog #AAA261715) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA261715 ELISA Kit recognizes Mouse MADCAM1. AAA Biotech's MADCAM-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Sandwich ELISA). Researchers should empirically determine the suitability of the MADCAM1 madcam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MADCAM-1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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