Angiotensin-converting enzyme 2 (ACE2) Active Protein | ACE2 active protein

Recombinant Human Angiotensin-converting enzyme 2 (ACE2), partial

Cat. Num. AAA244035

• Gene Names: ACE2; ACEH
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Angiotensin-converting enzyme 2 (ACE2); N/A; Recombinant Human Angiotensin-converting enzyme 2 (ACE2), partial; Angiotensin-converting enzyme 2; ACE2 active protein

• Host: Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.; Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH8.0; Lyophilized or liquid (Format to be determined during the manufa
• Sequence Positions: 18-740, Partial
• Sequence: QSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

• Species: Human
• Endotoxin: Less than 1.0EU/ug as determined by LAL method.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2ug/ml can bind Biotinylated human ACE2, the EC50 is 4.599-8.322ng/ml.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C.; Store working aliquots at 4 degrees C for up to one week.; Repeated freezing and thawing is not recommended.

Bioactivity

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

NCBI and Uniprot Product Information

• NCBI GI #: 11225609
• NCBI GeneID: 59272
• NCBI Accession #: NP_068576.1
• NCBI GenBank Nucleotide #: NM_021804.2
• UniProt Accession #: Q9BYF1; Q6UWP0; Q86WT0; Q9NRA7; Q9UFZ6; C7ECU1
• Molecular Weight: 92,463 Da
• NCBI Official Full Name: angiotensin-converting enzyme 2
• NCBI Official Synonym Full Names: angiotensin I converting enzyme 2
• NCBI Official Symbol: ACE2
• NCBI Official Synonym Symbols: ACEH
• NCBI Protein Information: angiotensin-converting enzyme 2; peptidyl-dipeptidase A; metalloprotease MPROT15; ACE-related carboxypeptidase; angiotensin-converting enzyme homolog; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
• UniProt Protein Name: Angiotensin-converting enzyme 2
• UniProt Gene Name: ACE2
• UniProt Synonym Gene Names: ACEH
• UniProt Entry Name: ACE2_HUMAN