Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2?g/mL can bind Anti-IL2RA recombinant antibody, the EC50 is 2.463-3.353 ng/mL.)
Interleukin-2 receptor subunit alpha (IL2RA) Active Protein | IL2RA active protein
Recombinant Human Interleukin-2 receptor subunit alpha (IL2RA), partial (Active)
Gene Names
IL2RA; CD25; IL2R; TCGFR; IDDM10
Purity
Greater than 95% as determined by SDS-PAGE.
Synonyms
Interleukin-2 receptor subunit alpha (IL2RA); N/A; Recombinant Human Interleukin-2 receptor subunit alpha (IL2RA), partial (Active); IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA; TAC antigen; p55; CD25; IL2RA active protein
Host
Mammalian Cell
Purity/Purification
Greater than 95% as determined by SDS-PAGE.
Form/Format
Lyophilized powder
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Lyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
Sequence Positions
22-213aa; Partial
Sequence
ELCDDDPPEIPHATFKAMAYKEGTMLNCECKRGFRRIKSGSLYMLCTGNSSHSSWDNQCQCTSSATRNTTKQVTPQPEEQKERKTTEMQSPMQPVDQASLPGHCREPPPWENEATERIYHFVVGQMVYYQCVQGYRALHRGPAESVCKMTHGKTRWTQPQLICTGEMETSQFPGEEKPQASPEGRPESETSC$
Species
Homo sapiens (Human)
Tag
C-terminal 10xHis-tagged
Endotoxin
Less than 1.0 EU/ug as determined by LAL method.
Research Area
Cancer
Biological Activity
1. Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 ug/mL can bind Anti-IL2RA recombinant antibody, the EC50 is 2.463-3.353 ng/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2 ug/mL can bind Human IL2, the EC50 is 1.693-2.039 ng/mL.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2?g/mL can bind Anti-IL2RA recombinant antibody, the EC50 is 2.463-3.353 ng/mL.)
Bioactivity
(Measured by its binding ability in a functional ELISA. Immobilized Human IL2RA at 2?g/mL can bind Anti-IL2RA recombinant antibody, the EC50 is 2.463-3.353 ng/mL.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
SDS-PAGE
((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)
Related Product Information for IL2RA active protein
Involve in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity.
Product Categories/Family for IL2RA active protein
References
"Molecular cloning of cDNA encoding human interleukin-2 receptor." Nikaido T., Shimizu A., Ishida N., Sabe H., Teshigawara K., Maeda M., Uchiyama T., Yodoi J., Honjo T. Nature 311:631-635 (1984)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,819 Da
NCBI Official Full Name
interleukin-2 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 2 receptor, alpha
NCBI Official Symbol
IL2RA
NCBI Official Synonym Symbols
CD25; IL2R; TCGFR; IDDM10
NCBI Protein Information
interleukin-2 receptor subunit alpha; p55; IL2-RA; IL-2-RA; TAC antigen; IL-2R subunit alpha; IL-2 receptor subunit alpha
UniProt Protein Name
Interleukin-2 receptor subunit alpha
UniProt Gene Name
IL2RA
UniProt Synonym Gene Names
IL-2 receptor subunit alpha; IL-2-RA; IL-2R subunit alpha; IL2-RA
UniProt Entry Name
IL2RA_HUMAN
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Product Notes
The IL2RA il2ra (Catalog #AAA279214) is an Active Protein produced from Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-213aa; Partial. The amino acid sequence is listed below: ELCDDDPPEI PHATFKAMAY KEGTMLNCEC KRGFRRIKSG SLYMLCTGNS SHSSWDNQCQ CTSSATRNTT KQVTPQPEEQ KERKTTEMQS PMQPVDQASL PGHCREPPPW ENEATERIYH FVVGQMVYYQ CVQGYRALHR GPAESVCKMT HGKTRWTQPQ LICTGEMETS QFPGEEKPQA SPEGRPESET SC$. It is sometimes possible for the material contained within the vial of "Interleukin-2 receptor subunit alpha (IL2RA), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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