Membrane Cofactor (CD46) Active Protein | CD46 active protein

Recombinant Human Membrane Cofactor Protein (CD46)

Cat. Num. AAA243727

• Gene Names: CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Membrane Cofactor (CD46); N/A; Recombinant Human Membrane Cofactor Protein (CD46); TLX; Trophoblast leukocyte common antigen; CD46; CD46 active protein

• Host: Mammalian cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 um filtered PBS, 6% Trehalose, pH 7.4.
• Sequence Positions: 35-328aa; Partial
• Sequence: CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
• Sequence Length: 392

• Species: Human
• Tag: C-terminal FC-tagged
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized CD46 at 2 ug/ml can bind Anti-CD46 rabbit monoclonal antibody, the EC50 of human CD46 protein is 0.8333-1.054 ng/ml.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Bioactivity

SDS-PAGE

((Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.)

Related Product Information for CD46 active protein

Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity.
(Microbial infection) A number of viral and bacterial pathogens seem to bind MCP in order to exploit its immune regulation property and directly induce an immunosuppressive phenotype in T-cells.
(Microbial infection) Acts as a receptor for Adenovirus subgroup B2 and Ad3.
(Microbial infection) Acts as a receptor for cultured Measles virus.
(Microbial infection) Acts as a receptor for Herpesvirus 6/HHV-6.
(Microbial infection) May act as a receptor for pathogenic bacteria Neisseria and Streptococcus pyogenes (PubMed:7708671, PubMed:9379894, PubMed:11260136, PubMed:11971006).

Product Categories/Family for CD46 active protein

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 41019474
• NCBI GeneID: 4179
• UniProt Accession #: P15529
• Molecular Weight: 61.6 kDa
• NCBI Official Full Name: Membrane cofactor protein
• NCBI Official Synonym Full Names: CD46 molecule
• NCBI Official Symbol: CD46
• NCBI Official Synonym Symbols: MCP; TLX; AHUS2; MIC10; TRA2.10
• NCBI Protein Information: membrane cofactor protein
• UniProt Protein Name: Membrane cofactor protein
• UniProt Gene Name: CD46
• UniProt Synonym Gene Names: MCP; MIC10
• UniProt Entry Name: MCP_HUMAN