Application Data
(MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
MAOB recombinant protein
Recombinant MAOB protein
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
MAOB; N/A; Recombinant MAOB protein; Monoamine Oxidase B; MAOB recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Full length recombinant MAOB protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 5% Glycerol, 0.04% Triton X-100, 0.2mM TCEP, 0.2mg/ml 3× DYKDDDDK peptide.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant MAOB protein is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. Monoamine Oxidase Assay: 40uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
Application Data
(MAO-GloTM Assay for MAOA activity 40 uM substrate was incubated with MAOA protein in reaction buffer for 1 hour at room temperature, then the product was incubated with Detection Reagent for 0.5 hour at temperature. The activity of MAOA was detected by Promega MAO-Glo Assay.)
SDS-PAGE
(Recombinant MAOB protein gel. MAOB protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
SDS-PAGE
(Recombinant MAOB protein gel. MAOB protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Related Product Information for MAOB recombinant protein
Short Description: Recombinant MAOB (accession number NP_000889.3) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK-Tag with an observed molecular weight of 59.8 kDa. The recombinant protein is >85% pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Background: MAOB (Monoamine Oxidase B) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOA. MAOB preferentially degrades benzylamine and phenylethylamine. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues.
Background: MAOB (Monoamine Oxidase B) is one of two neighboring mitochondrial enzyme family members which catalyze the oxidative deamination of amines, such as dopamine, norepinephrine, and serotonin. The other member is MAOA. MAOB preferentially degrades benzylamine and phenylethylamine. MAOB catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues.
Product Categories/Family for MAOB recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,539 Da
NCBI Official Full Name
amine oxidase
NCBI Official Synonym Full Names
monoamine oxidase B
NCBI Official Symbol
MAOB
NCBI Protein Information
amine oxidase [flavin-containing] B; amine oxidase [flavin-containing] B; MAO, brain; MAO, platelet; MAO-B; adrenalin oxidase; monoamine oxidase type B; tyramine oxidase
UniProt Protein Name
Amine oxidase [flavin-containing] B
UniProt Gene Name
MAOB
UniProt Synonym Gene Names
MAO-B
UniProt Entry Name
AOFB_HUMAN
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Product Notes
The MAOB maob (Catalog #AAA59724) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MAOB, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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