Application Data
GLK recombinant protein
GLK, Active
Gene Names
MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
Applications
Western Blot
Synonyms
GLK; N/A; GLK, Active; GLK recombinant protein
Host
Sf9 insect cells using baculovirus
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Positions
1-380
Sequence Length
4362
Applicable Applications for GLK recombinant protein
WB (Western Blot), Kinase Assay
Species
Human
Tag
GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
Application Data
Application Data
Application Data
Related Product Information for GLK recombinant protein
Description: Recombinant human GLK (1-380) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-ï¡ and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-theta during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-theta and the kinase IKK in T cells (1). GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM (2).
Scientific Background: GLK is a member of the STE20 family of serine/threonine protein kinases and contains N-terminal catalytic domain and C-terminal regulatory domain. GLK is upstream of the MEKK1 target and is stimulated by UV radiation and TNF-ï¡ and can lead to the activation of the JNK signaling pathway. GLK can directly activate PKC-theta during TCR signaling and people with systemic lupus erythematosus show considerable enhanced GLK expression and activation of PKC-theta and the kinase IKK in T cells (1). GLK can act as a pro-apoptotic kinase which orchestrates activation of BAX via the concerted posttranscriptional modulation of PUMA, BAD, and BIM (2).
Product Categories/Family for GLK recombinant protein
References
1. Chuang, H C. et al: The kinase GLK controls autoimmunity and NF-kappaB signaling by activating the kinase PKC-theta in T cells. Nat Immunol. 2011 Oct 9;12 (11):1113-8. 2. Lam, D. et al: MAP4K3 modulates cell death via the post-transcriptional regulation of BH3-only proteins. Proc Natl Acad Sci U S A. 2009 Jul 21;106 (29):11978-83.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~70 kDa
NCBI Official Full Name
Homo sapiens mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3), transcript variant 1, mRNA
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol
MAP4K3
NCBI Official Synonym Symbols
GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 3
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 3
UniProt Gene Name
MAP4K3
UniProt Synonym Gene Names
RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
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Product Notes
The GLK map4k3 (Catalog #AAA73015) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-380. AAA Biotech's GLK can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the GLK map4k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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