HGK recombinant protein

HGK, Active

Cat. Num. AAA73672

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Applications: Western Blot
• Synonyms: HGK; N/A; HGK, Active; MAP4K4; NIK; FLH21957; FLJ10410; FLJ20373; FLJ90111; KIAA0687; HGK recombinant protein

• Host: Sf9 insect cells
• Form/Format: Recombinant protein stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

• Applicable Applications for HGK recombinant protein: WB (Western Blot), Kinase Assay
• Tag: GST
• Species: Human
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)

Activity

(Specific Activity: Sample Kinase Activity Plot. For specific information on a given lot, see related technical data sheet.)

Related Product Information for HGK recombinant protein

Recombinant human HGK (1-328) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The protein accession number is NP_004825.
HGK is the mitogen-activated protein kinase kinase kinase kinase 4(MAP4K4) and a member of the serine/threonine protein kinase family. HGK has been shown to specifically activate MAPK8/JNK (1). The activation of MAPK8 by HGK can be inhibited by dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggest that this kinase functions through the MAP3K7-MAP2K4-MAP2K7 kinase cascade and mediates TNF-alpha signaling. HGK-dependent signaling inhibits PPARgamma-responsive gene expression, adipogenesis, and insulin-stimulated glucose transport (2).

Product Categories/Family for HGK recombinant protein

Protein Kinases; Apoptosis/Autophagy; Cardiovascular Disease; Cellular Stress; Inflammation; JNK/SAPK Pathway; Neurobiology; NfkB Pathway; Ser/Thr Kinases

References

1. Yao, Z. et.al: A novel human STE20-related protein kinase, HGK, that specifically activates the c-jun N-terminal kinase signaling pathway. J. Biol. Chem. 274: 2118-2125, 1999.
2. Tang, X. et.al: An RNA interference-based screen identifies MAP4K4/NIK as a negative regulator of PPAR-gamma, adipogenesis, and insulin-responsive hexose transport. Proc. Nat. Acad. Sci. 103: 2087-2092, 2006.

NCBI and Uniprot Product Information

• NCBI GI #: 336020353
• NCBI GeneID: 9448
• NCBI Accession #: NP_004825
• NCBI GenBank Nucleotide #: NM_004834
• UniProt Accession #: O95819
• Molecular Weight: 142kDa
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN