WB (Western Blot)
(Detection of human MAP4K4/HGK by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAP4K4/HGK antibody AAA211506 (lot AAA211506-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human MAP4K4/HGK Polyclonal Antibody | anti-MAP4K4/HGK antibody
Rabbit anti-MAP4K4/HGK Antibody, Affinity Purified
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MAP4K4/HGK, Antibody; Rabbit anti-MAP4K4/HGK Antibody, Affinity Purified; MEK kinase kinase 4; Ste20 group protein kinase HGK; NIK; MEKKK4; mitogen-activated protein kinase kinase kinase kinase 4; MEKKK 4; HPK/GCK-like kinase HGK; HGK; hepatocyte progenitor kinase-like/germinal center kinase-like kinase; HEL-S-31; FLH21957; epididymis secretory protein Li 31; nck-interacting kinase; MAPK/ERK kinase kinase kinase 4; Mitogen-activated protein kinase kinase kinase kinase 4; anti-MAP4K4/HGK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MAP4K4/HGK antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Mouse
Immunogen
Between 875 and 925
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MAP4K4/HGK by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAP4K4/HGK antibody AAA211506 (lot AAA211506-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human MAP4K4/HGK by western blot. Samples: Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAP4K4/HGK antibody AAA211506 (lot AAA211506-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human MAP4K4/HGK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP4K4/HGK antibody AAA211506 (lot AAA211506-2) used for IP at 6 ug per reaction. MAP4K4/HGK was also immunoprecipitated by a previous lot of this antibody (AAA211506-1) and rabbit anti-MAP4K4/HGK antibody For blotting immunoprecipitated MAP4K4/HGK, AAA211506 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human MAP4K4/HGK by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAP4K4/HGK antibody AAA211506 (lot AAA211506-2) used for IP at 6 ug per reaction. MAP4K4/HGK was also immunoprecipitated by a previous lot of this antibody (AAA211506-1) and rabbit anti-MAP4K4/HGK antibody For blotting immunoprecipitated MAP4K4/HGK, AAA211506 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Related Product Information for anti-MAP4K4/HGK antibody
MAP4K4 is a serine/threonine kinase that signals upstream of the c-Jun N-terminal kinase (JNK) and is involved in cell motility. MAP4K4 appears to partially mediate TNF alpha signaling. In skeletal muscle TNF-alpha induces insulin resistance. TNF-alpha induced insulin resistance has been shown to be dependent on MAPK4K4 activity. Interestingly, TNF-alpha regulates MAP4K4 expression and can stimulate MAP4K4 gene expression via TNFR2 (TNFalpha receptor 2) and the c-Jun and ATF2 (activating transcription factor 2) transcription factors. MAP4K4 is highly expressed in most tumor cell lines, and the analysis of MAP4K4 mutants indicates that it may have an important role in cell transformation and invasiveness.
Product Categories/Family for anti-MAP4K4/HGK antibody
NCBI and Uniprot Product Information
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Product Notes
The MAP4K4/HGK map4k4 (Catalog #AAA211506) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MAP4K4/HGK Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4K4/HGK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MAP4K4/HGK map4k4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4K4/HGK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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