Human Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 ELISA Kit | MAP4K5 elisa kit

Human Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5) (Sandwich ELISA) ELISA Kit

Cat. Num. AAA190078

• Gene Names: MAP4K5; KHS; GCKR; KHS1; MAPKKKK5
• Reactivity: Human
• Synonyms: Mitogen Activated Protein Kinase Kinase Kinase Kinase 5; N/A; Human Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5) (Sandwich ELISA) ELISA Kit; MAP4-K5; GCKR; KHS; KHS1; MAPKKKK5; Germinal Center Kinase-Related; Kinase homologous to SPS1/STE20; ELISA Kit for Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5); MAP4K5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of MAP4K5. No significant cross-reactivity or interference between MAP4K5 and analogues was observed.
• Assay Type: Quantitative Sandwich
• Samples: Human tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.119ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MAP4K5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MAP4K5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: 4 degree C for 1 month/ -20 degree C for long-term(within shelf life). Shelf life is one year

Standard Curve (Sample)

Related Product Information for MAP4K5 elisa kit

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to MAP4K5. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to MAP4K5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAP4K5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAP4K5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MAP4K5 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Product Categories/Family for MAP4K5 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 38570135
• NCBI GeneID: 11183
• NCBI Accession #: NP_942089.1
• NCBI GenBank Nucleotide #: NM_198794.2
• UniProt Accession #: Q9Y4K4; Q8IYF6; A0A0A0MQR1
• Molecular Weight: 95,024 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 5
• NCBI Official Symbol: MAP4K5
• NCBI Official Synonym Symbols: KHS; GCKR; KHS1; MAPKKKK5
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 5
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 5
• UniProt Gene Name: MAP4K5
• UniProt Synonym Gene Names: KHS; MEK kinase kinase 5; MEKKK 5