Truncated Tau (AA297-391)(dGAE C322A) Recombinant Protein | dGAE recombinant protein

Human Recombinant Truncated Tau (AA297-391)(dGAE C322A) Protein Pre-formed Fibrils

Cat. Num. AAA254092

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• Applications: SDS-Page, Western Blot
• Purity: >95%; Ion-exchange Purified
• Synonyms: Truncated Tau (AA297-391)(dGAE C322A); N/A; Human Recombinant Truncated Tau (AA297-391)(dGAE C322A) Protein Pre-formed Fibrils; Tau Truncated (AA297-391)(dGAE C322A) Pre-formed Fibrils; Truncated Tau Fragment (AA297-391)(dGAE); Tau aggregate; Tau PFFs; Tau PFF; Tau protein aggregate; Tau protein; microtubule-associated protein Tau; MAPT; MAP; microtubule-associated protein; Truncated Tau Protein Aggregate; Paired Helical Filament-Tau; Phf-Tau; Neurofibrillary Tangle Protein; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; Isoform 2; tubulin-associated unit; 95-amino acid Tau protein fragment; Truncated Tau Protein; dGAE recombinant protein

• Host: E coli
• Purity/Purification: >95%; Ion-exchange Purified
• Form/Format: PBS pH 7.4
• Concentration: Lot/batch specific. See included datasheet. (varies by lot)
• Sequence Length: Fragment

• Applicable Applications for dGAE recombinant protein: Invitro Assay, SDS-PAGE, WB (Western Blot)
• Species: Human
• Conjugation: No tag
• Research Area: Alzheimer's Disease, Axon Markers, Cell Markers, Cell Signaling, Cytoskeleton, Microtubules, MT Associated Proteins, Neurodegeneration, Neuron Markers, Neuroscience, Tangles & Tau
• Cellular Localization: Cytoplasm, Axolemma, Axolemma Plasma Membrane, Axon, Cell Body, Cell membrane, Cytoplasmic Ribonucleoprotein Granule, Cytoplasmic Side, Cytoskeleton, Cytosol, Dendrite, Growth cone, Microtubule, Microtubule Associated Complex, Neurofibrillary Tangle, Neuronal Cell Body, Nuclear Periphery, Nuclear Speck, Nucleus, Peripheral membrane protein, Plasma Membrane, Tubulin Complex
• Biological Activity: Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) over time when truncated tau fragment (AA297-391)(dGAE C322A) monomer is combined with truncated tau fragment (AA297-391)(dGAE C322A) Pre-formed Fibrils.
• Certificate of Analysis: Certified >95% pure using SDS-PAGE analysis. Low endotoxin <5 EU/mL @ 2mg/mL.
• Other Relevant Information: For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or on the product datasheet for further information.
• Preparation and Storage: Store at -80 degree C

Application Data

(ThioflavinTisafluorescentdyethatbindstobetasheet-richstructures,suchasthoseintaufibrils.Uponbinding,theemissionspectrumofthedyeexperiencesared-shiftandincreasedfluorescenceintensity.ThioflavinTemissioncurvesshowincreasedfluorescence(correlatedtotauaggregation)overtimewhentruncatedtaufragment(AA297-391)(dGAEC322A)monomeriscombinedwithtruncatedtaufragment(AA297-391)(dGAEC322A)Pre-formedFibrils(Type1).)

Application Data

(TEMofActiveHumanRecombinantTruncatedTau(AA297-391)(dGAEC322A)ProteinPre-formedFibrils(Type1)(AAA254092))

Related Product Information for dGAE recombinant protein

Alzheimer's Disease (AD) is the most common neurodegenerative disease, affecting 10% of seniors over the age of 65 (1). It was named after Alois Alzheimer, a German scientist who discovered tangled bundles of fibrils where neurons had once been in the brain of a deceased patient in 1907 (2). Tau (tubulin-associated unit) is normally located in the axons of neurons where it stabilizes microtubules. Tauopathies such as AD are characterized by neurofibrillary tangles containing paired helical filaments (PHFs). A truncated 95-amino acid fragment corresponding to residues 297-391 of full-length tau has been shown to assemble into PHF-like fibrils in vitro in the absence of additives or templates (3). This fragment has been found in the core of PHFs from AD brains and forms filaments that closely resemble PHFs isolated from AD brains (3). The C322A mutation leads to enhanced self-assembly into long and ordered PHFs (3).

Product Categories/Family for dGAE recombinant protein

Proteins

References

1. www.alz.org/alzheimers-dementia/facts-figures2. Alzheimer, A. Über eine eigenartige Erkrankung der Hirnrinde. Allg. Z. Psychiatr. Psych.-Gerichtl. Med. 64, 146-148 (1907)3. Al-Hilaly, Y.K. et al. Alzheimer's Disease-like Paired Helical Filament Assembly from Truncated Tau Protein Is Independent of Disulfide Crosslinking. J. Mol. Biol. 429(23):3650-3665 (2017)

NCBI and Uniprot Product Information

• NCBI GI #: 6754638
• NCBI GeneID: 4137
• NCBI Accession #: NP_005901.2
• NCBI GenBank Nucleotide #: NM_005910.5
• UniProt Accession #: P10636; P18518; Q14799; Q15549; Q15550; Q15551; Q1RMF6; Q53YB1; Q5CZI7; Q5XWF0; Q6QT54; Q9UDJ3
• Molecular Weight: 80,941 Da
• NCBI Official Full Name: microtubule-associated protein tau isoform 2
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
• NCBI Protein Information: microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; protein phosphatase 1, regulatory subunit 103; G protein beta1/gamma2 subunit-interacting factor 1
• UniProt Protein Name: Microtubule-associated protein tau
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU; PHF-tau
• UniProt Entry Name: TAU_HUMAN