Chicken Myoglobin ELISA Kit | Mb elisa kit

Chicken Myoglobin ELISA Kit

Cat. Num. AAA16786

• Gene Names: MB; PVALB
• Reactivity: Chicken
• Synonyms: Myoglobin; N/A; Chicken Myoglobin ELISA Kit; Mb elisa kit

• Reactivity: Chicken
• Specificity: This assay has high sensitivity and excellent specificity for detection of MB. No significant cross-reactivity or interference between MB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MB and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C

Standard Curve (Sample)

Related Product Information for Mb elisa kit

Principle of the Assay: MB ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MB antibody and an MB-HRP conjugate. The assay sample and buffer are incubated together with MB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MB concentration since MB from samples and MB-HRP conjugate compete for the anti-MB antibody binding site. Since the number of sites is limited, as more sites are occupied by MB from the sample, fewer sites are left to bind MB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MB concentration in each sample is interpolated from this standard curve.

Intended Uses: This MB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken MB. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for Mb elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 44955888
• NCBI GeneID: 4151
• NCBI Accession #: NP_976312.1
• NCBI GenBank Nucleotide #: NM_203378.1
• UniProt Accession #: P02144; Q52H51; Q5THY7; Q8WVH6
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN