Application Data
MBP recombinant protein
MBP Protein
Applications
Western Blot
Purity
>90%
Synonyms
MBP; N/A; MBP Protein; MBP recombinant protein
Purity/Purification
>90%
Form/Format
Native protein stored in 100mM MOPS, pH 6.5 and 0.1mM PMSF.
Concentration
1 ug/ul (varies by lot)
Applicable Applications for MBP recombinant protein
WB (Western Blot), Kinase Assay
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -20 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Stability: 1yr at -20 degree C from date of shipment.
Shipping: Ships with Dry Ice
Stability: 1yr at -20 degree C from date of shipment.
Shipping: Ships with Dry Ice
Application Data
Application Data
Application Data
Application Data
Related Product Information for MBP recombinant protein
Native Swine MBP was extracted under acidic conditions and further purified by cation chromatography from pig brain (1). This product is routinely evaluated using active MAP Kinase 3/ERK1.
Scientific Background: MBP exists as four major forms in the pig CNS with apparent molecular weights of 21.5K, 20.2K, 18.5K, and 17.3K (2). Native pig MBP is extracted under acidic conditions and further purified by cation chromatography. MBP is an efficient substrate for numerous protein kinases including ERK/MAP Kinase family, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase (3, 4).
Scientific Background: MBP exists as four major forms in the pig CNS with apparent molecular weights of 21.5K, 20.2K, 18.5K, and 17.3K (2). Native pig MBP is extracted under acidic conditions and further purified by cation chromatography. MBP is an efficient substrate for numerous protein kinases including ERK/MAP Kinase family, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase (3, 4).
References
1. Chevalier D, et al. Purification of myelin basic protein from bovine brain. Protein Expr Purif. 18(2):229-34, 2000.
2. Sheng HZ, et al. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40.
3. Sanghera J. et al. Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk. FEBS Lett. 1990 Oct 29;273(1-2):223-6.
4. Martenson, et al., Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein. J. Biol. Chem. 258: 930, 1983.
2. Sheng HZ, et al. Developmental study of myelin basic protein variants in various regions of pig nervous system. J Neurochem. 1989 Mar;52(3):736-40.
3. Sanghera J. et al. Identification of the sites in myelin basic protein that are phosphorylated by meiosis-activated protein kinase p44mpk. FEBS Lett. 1990 Oct 29;273(1-2):223-6.
4. Martenson, et al., Identification of multiple in vivo phosphorylation sites in rabbit myelin basic protein. J. Biol. Chem. 258: 930, 1983.
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Product Notes
The MBP (Catalog #AAA72939) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MBP can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Kinase Assay. Researchers should empirically determine the suitability of the MBP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MBP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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