Human Meprin A subunit alpha ELISA Kit | MEP1A elisa kit

Human Meprin A subunit alpha ELISA Kit

Cat. Num. AAA98125

• Gene Names: MEP1A; PPHA
• Reactivity: Human
• Synonyms: Meprin A subunit alpha; N/A; Human Meprin A subunit alpha ELISA Kit; MEP1A/PPHA/Endopeptidase-2/PPH alpha/meprin A subunit alpha/meprin A; alpha (PABA peptide hydrolase)/N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha/PABA peptide hydrolase/bA268F1.1 (meprin A alpha (PABA peptide hydrolase)); MEP1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection ofMdk. No significant cross-reactivity or interference betweenMdk and analogues was observed.
• Sequence Length: 746
• Assay Type: Sandwich ELISA, Double Antibody
• Samples: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelMdk were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelMdk were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C

Standard Curve (Sample)

Related Product Information for MEP1A elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mdk antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mdk antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mdk amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMdk can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 153070262
• NCBI GeneID: 4224
• NCBI Accession #: NP_005579.2
• NCBI GenBank Nucleotide #: NM_005588.2
• Molecular Weight: 84,419 Da
• NCBI Official Full Name: meprin A subunit alpha
• NCBI Official Synonym Full Names: meprin A subunit alpha
• NCBI Official Symbol: MEP1A
• NCBI Official Synonym Symbols: PPHA
• NCBI Protein Information: meprin A subunit alpha
• UniProt Protein Name: Meprin A subunit alpha
• UniProt Gene Name: MEP1A
• UniProt Entry Name: MEP1A_HUMAN