c-MET / HGFR recombinant protein

Recombinant Mouse c-MET / HGFR Protein (His tag)

Cat. Num. AAA22100

• Gene Names: Met; HGF; HGFR; Par4; c-Met; AI838057
• Purity: > 90 % as determined by reducing SDS-PAGE
• Synonyms: c-MET / HGFR; N/A; Recombinant Mouse c-MET / HGFR Protein (His tag); AI838057;c-Met;HGF;HGFR;Par4; c-MET / HGFR recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 90 % as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence: Met 1-Asn 929
• Sequence Length: 1379

• Tag: C-His
• Species: Mouse
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Bioactivity: Immobilized HGF Protein, Mouse, Recombinant at 2 ug/mL (100 uL/well) can bind c-MET Protein, Mouse, Recombinant (ECD, His Tag, the EC50 is 80-400 ng/mL.
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex.; It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 degrees C to -80 degrees C . Reconstituted protein solution can be stored at 4-8 degrees C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degrees C for 3 months.

SDS-PAGE

(> 90% as determined by reducing SDS-PAGE)

Related Product Information for c-MET / HGFR recombinant protein

Background: Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotype s. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

NCBI and Uniprot Product Information

• NCBI GI #: 146198696
• NCBI GeneID: 17295
• NCBI Accession #: EDL13881.1
• NCBI GenBank Nucleotide #: NM_008591.2
• UniProt Accession #: P16056; Q62125
• Molecular Weight: Calculated molecular weight 102 kDa; Observed molecular weight 41.8&87.2 kDa
• NCBI Official Full Name: hepatocyte growth factor receptor
• NCBI Official Synonym Full Names: met proto-oncogene
• NCBI Official Symbol: Met
• NCBI Official Synonym Symbols: HGF; HGFR; Par4; c-Met; AI838057
• NCBI Protein Information: hepatocyte growth factor receptor
• UniProt Protein Name: Hepatocyte growth factor receptor
• UniProt Gene Name: Met
• UniProt Synonym Gene Names: HGF receptor; SF receptor