Bioactivity
(The specific activity of METTL3/METTL14 was determined to be 750 pmol/min/mg as per activity assay protocol.)
METTL3/METTL14 recombinant protein
METTL3/METTL14, Active
Purity
>90%
Synonyms
METTL3/METTL14; N/A; METTL3/METTL14, Active; METTL3: IME4; M6A; MT-A70; Spo8 METTL14: None; METTL3/METTL14 recombinant protein
Host
Sf9 insect cells
Purity/Purification
>90%
Form/Format
Recombinant proteins stored in 50mM Tris-HCl, pH7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.1 ug/ul (varies by lot)
Tag
GST
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Bioactivity
(The specific activity of METTL3/METTL14 was determined to be 750 pmol/min/mg as per activity assay protocol.)
Bioactivity
(The specific activity of METTL3/METTL14 was determined to be 750 pmol/min/mg as per activity assay protocol.)
SDS-PAGE
(The purity of METTL3/METTL14 complex was determined to be >90% by densitometry. METTL3 approx. MW 100 kDa.METTL14 approx. MW 84 kDa.)
SDS-PAGE
(The purity of METTL3/METTL14 complex was determined to be >90% by densitometry. METTL3 approx. MW 100 kDa.METTL14 approx. MW 84 kDa.)
Related Product Information for METTL3/METTL14 recombinant protein
Full-length recombinant human METTL3/METTL14 complex was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The METTL3 and METTL14 gene accession numbers are NM_019852 and NM_020961.
Methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) form a stable heterodimer core complex that catalyzes N6-methyladenosine (m6A) RNA methylation in mammalian cells. m6A is an abundant internal modification in messenger RNA and long non-coding RNA. It functions in multiple aspects of developmental regulation, cell cycle progression, cell fate, and the heat shock stress response by affecting aspects of RNA metabolism such as pre-mRNA processing, translation efficiency, transcript stability and miRNA biogenesis.
Methyltransferase-like 3 (METTL3) and methyltransferase-like 14 (METTL14) form a stable heterodimer core complex that catalyzes N6-methyladenosine (m6A) RNA methylation in mammalian cells. m6A is an abundant internal modification in messenger RNA and long non-coding RNA. It functions in multiple aspects of developmental regulation, cell cycle progression, cell fate, and the heat shock stress response by affecting aspects of RNA metabolism such as pre-mRNA processing, translation efficiency, transcript stability and miRNA biogenesis.
Product Categories/Family for METTL3/METTL14 recombinant protein
References
1. Liu J. et al: A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation. Nat. Chem. Biol. 2014; 10: 93-95
2. Wang X. et al: Structural basis of N6-adenosine methylation by the METTL3-METTL14 complex. Nature 2016; 534:575-578
3. Wang P. et al: Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases. Mol. Cell 2016; 63(2):306-317
2. Wang X. et al: Structural basis of N6-adenosine methylation by the METTL3-METTL14 complex. Nature 2016; 534:575-578
3. Wang P. et al: Structural Basis for Cooperative Function of Mettl3 and Mettl14 Methyltransferases. Mol. Cell 2016; 63(2):306-317
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Similar Products
Product Notes
The METTL3/METTL14 (Catalog #AAA73676) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "METTL3/METTL14, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.