Rat MHC class I polypeptide-related sequence B ELISA Kit | MICB elisa kit

Rat MHC class I polypeptide-related sequence B ELISA Kit

Cat. Num. AAA82978

• Gene Names: MICB; PERB11.2
• Reactivity: Rat
• Synonyms: MHC class I polypeptide-related sequence B; N/A; Rat MHC class I polypeptide-related sequence B ELISA Kit; MICB elisa kit

• Reactivity: Rat
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for MICB elisa kit

Intended Uses: This MHC I/H-2 III ELISA kit is intended Laboratory for research use only. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofMHC I/H-2 III in the sample, thisMHC I/H-2 III ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus MHC I/H-2 III concentration. The concentration of MHC I/H-2 III in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This MHC I/H-2 III enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MHC I/H-2 III. Standards or samples are then added to the microtiter plate wells and MHC I/H-2 III if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MHC I/H-2 III present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MHC I/H-2 III are added to each well to "sandwich" the MHC I/H-2 III immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MHC I/H-2 III and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

Product Categories/Family for MICB elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 167857788
• NCBI GeneID: 4277
• NCBI Accession #: NP_005922.2
• NCBI GenBank Nucleotide #: NM_005931.4
• Molecular Weight: 4,287 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence B isoform 1
• NCBI Official Synonym Full Names: MHC class I polypeptide-related sequence B
• NCBI Official Symbol: MICB
• NCBI Official Synonym Symbols: PERB11.2
• NCBI Protein Information: MHC class I polypeptide-related sequence B; MHC class I mic-B antigen; stress inducible class I homolog; MHC class I chain-related protein B; MHC class I-like molecule PERB11.2-IMX
• UniProt Protein Name: MHC class I polypeptide-related sequence B
• UniProt Gene Name: MICB
• UniProt Synonym Gene Names: PERB11.2; MIC-B
• UniProt Entry Name: MICB_HUMAN