Standard Curve (Sample)
Mouse Macrophage Inflammatory Protein 1a ELISA Kit | MIP1a elisa kit
Mouse Macrophage Inflammatory Protein 1a ELISA Kit
Gene Names
CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
Reactivity
Mouse
Synonyms
Macrophage Inflammatory Protein 1a; N/A; Mouse Macrophage Inflammatory Protein 1a ELISA Kit; MIP1a elisa kit
Reactivity
Mouse
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
5.0-100ng/mL
Sensitivity
1.0ng/mL
Highest Standards
100ng/mL
Concentration of Standards
Concentration of Standards
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for MIP1a elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This MIP1A/CCL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MIP1A/CCL3. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY MIP1A/CCL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP1A/CCL3 antibody and an MIP1A/CCL3-HRP conjugate. The assay sample and buffer are incubated together with MIP1A/CCL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP1A/CCL3 concentration since MIP1A/CCL3 from samples and MIP1A/CCL3-HRP conjugate compete for the anti-MIP1A/CCL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP1A/CCL3 from the sample, fewer sites are left to bind MIP1A/CCL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1A/CCL3 concentration in each sample is interpolated from this standard curve.
INTENDED USE This MIP1A/CCL3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MIP1A/CCL3. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY MIP1A/CCL3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MIP1A/CCL3 antibody and an MIP1A/CCL3-HRP conjugate. The assay sample and buffer are incubated together with MIP1A/CCL3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MIP1A/CCL3 concentration since MIP1A/CCL3 from samples and MIP1A/CCL3-HRP conjugate compete for the anti-MIP1A/CCL3 antibody binding site. Since the number of sites is limited, as more sites are occupied by MIP1A/CCL3 from the sample, fewer sites are left to bind MIP1A/CCL3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MIP1A/CCL3 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MIP1a elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,085 Da
NCBI Official Full Name
macrophage inflammatory protein 1 alpha, partial
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 3
NCBI Official Symbol
CCL3
NCBI Official Synonym Symbols
MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha
NCBI Protein Information
C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha; tonsillar lymphocyte LD78 alpha protein; small inducible cytokine A3 (homologous to mouse Mip-1a)
UniProt Protein Name
C-C motif chemokine 3
UniProt Gene Name
CCL3
UniProt Synonym Gene Names
G0S19-1; MIP1A; SCYA3; MIP-1-alpha
UniProt Entry Name
CCL3_HUMAN
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Product Notes
The Mouse MIP1a ccl3 (Catalog #AAA86579) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA86579 ELISA Kit recognizes Mouse MIP1a. It is sometimes possible for the material contained within the vial of "Macrophage Inflammatory Protein 1a, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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