MMP-8 recombinant protein

Recombinant Human MMP-8 Protein

Cat. Num. AAA283402

• Purity: >95% as determined by Tris-Bis PAGE
• Synonyms: MMP-8; N/A; Recombinant Human MMP-8 Protein; PMNL-CL; CLG1; MMP8; Collagenase 2; HNC; MMP-8 recombinant protein

• Host: HEK293 cells
• Purity/Purification: >95% as determined by Tris-Bis PAGE
• Form/Format: Lyophilized from a 0.2 um filtered solution of 50mM Tris, 10 mM CaCl2, 150 mM NaCl, pH7.5. Contact us for customized product form or formulation.
• Sequence: FPVSSKEKNTKTVQDYLEKFYQLPSNQYQSTRKNGTNVIVEKLKEMQRFFGLNVTGKPNEETLDMMKKPRCGVPDSGGFMLTPGNPKWERTNLTYRIRNYTPQLSEAEVERAIKDAFELWSVASPLIFTRISQGEADINIAFYQRDHGDNSPFDGPNGILAHAFQPGQGIGGDAHFDAEETWTNTSANYNLFLVAAHEFGHSLGLAHSSDPGALMYPNYAFRETSNYSLPQDDIDGIQAIYGLSSNPIQPTGPSTPKPCDPSLTFDAITTLRGEILFFKDRYFWRRHPQLQRVEMNFISLFWPSLPTGIQAAYEDFDRDLIFLFKGNQYWALSGYDILQGYPKDISNYGFPSSVQAIDAAVFYRSKTYFFVNDQFWRYDNQRQFMEPGYPKSISGAFPGIESKVDAVFQQEHFFHVFSGPRYYAFDLIAQRVTRVARGNKWLNCRYG

• Species: Human
• Tag: C-His
• Endotoxin: <0.01EU/ug by the LAL method.
• Bio-Activity: Recombinant Human MMP-8 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RD #ES001). The specific activity is >194 pmol/min/ug, as measured under the described conditions.
• Preparation and Storage: Store at -20 degree C. Store the lyophilized protein at -20 degree C to -80 degree C up to 1 year from the date of receipt.; After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.

Application Data

(Recombinant Human MMP-8 Protein cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (RD#ES001). The specific activity is >194 pmol/min/ug, as measured under the described conditions.)

Application Data

(Anti-MMP-8 Antibody captured on CM5 Chip via Protein A can bind Human MMP-8, His Tag with an affinity constant of 1.14 uM as determined in SPR assay (Biacore T200).)

Application Data

(Immobilized Human MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC₅₀ of 3.5ug/ml determined by ELISA.)

Application Data

(The purity of Human MMP-8 is greater than 95% as determined by SEC-HPLC.)

SDS-PAGE

(Recombinant Human MMP-8 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 60-75kDa.)

Related Product Information for MMP-8 recombinant protein

Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Product Categories/Family for MMP-8 recombinant protein

Other Recombinant Protein; Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4505221
• NCBI GeneID: 4317
• NCBI Accession #: NP_002415.1
• NCBI GenBank Nucleotide #: NM_002424.2
• UniProt Accession #: P22894; Q45F99
• UniProt Protein Name: Neutrophil collagenase
• UniProt Gene Name: MMP8
• UniProt Synonym Gene Names: CLG1; MMP-8; PMNL-CL
• UniProt Entry Name: MMP8_HUMAN