WB (Western Blot)
(Detection of 4-HNE by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2 cells untreated (-) or treated with 100uM 4-HNE for 1hr. Antibody: Rabbit anti-4-HNE recombinant monoclonal antibody (AAA213700 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
4-HNE recombinant antibody
Rabbit anti-4-HNE Recombinant Monoclonal Antibody [BLR303M]
Applications
Western Blot
Purity
Purified
Synonyms
4-HNE, Recombinant Antibody; Rabbit anti-4-HNE Recombinant Monoclonal Antibody [BLR303M]; 4-HNE recombinant antibody
Host
Rabbit
Clonality
Monoclonal
Clone Number
BLR303M
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Phosphate Buffered Saline (PBS) with 0.09% Sodium Azide, BSA-Free
Concentration
1000ug/ml (varies by lot)
Applicable Applications for 4-HNE recombinant antibody
WB (Western Blot)
Immunogen
4-HNE
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of 4-HNE by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2 cells untreated (-) or treated with 100uM 4-HNE for 1hr. Antibody: Rabbit anti-4-HNE recombinant monoclonal antibody (AAA213700 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of 4-HNE by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2 cells untreated (-) or treated with 100uM 4-HNE for 1hr. Antibody: Rabbit anti-4-HNE recombinant monoclonal antibody (AAA213700 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot)
(Detection of 4-HNE by western blot. Samples: 1ug BSA conjugated to 4-HNE, Malondialdehyde (MDA), 4-Hydroxy-2-hexenal (4-HHE), Hexanoyl Lysine (H-Lys), and Crotonaldehyde (Croto). Antibody: Rabbit anti-4-HNE recombinant monoclonal antibody (AAA213700 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
WB (Western Blot)
(Detection of 4-HNE by western blot. Samples: 1ug BSA conjugated to 4-HNE, Malondialdehyde (MDA), 4-Hydroxy-2-hexenal (4-HHE), Hexanoyl Lysine (H-Lys), and Crotonaldehyde (Croto). Antibody: Rabbit anti-4-HNE recombinant monoclonal antibody (AAA213700 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for 4-HNE recombinant antibody
4-Hydroxynonenal (4-HNE) is normally found in the human body at low concentrations. At higher concentrations, it has been linked to the induction of apoptosis and necrosis and several diseases, ranging from Alzheimer's disease to cancer.
Product Categories/Family for 4-HNE recombinant antibody
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Product Notes
The 4-HNE (Catalog #AAA213700) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 4-HNE can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the 4-HNE for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-HNE, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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