Mouse anti-Human ABCB4 Monoclonal Antibody | anti-ABCB4 antibody

ABCB4 Antibody

Cat. Num. AAA290845

• Gene Names: ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Synonyms: ABCB4, Antibody; ABCB4 Antibody; Multidrug resistance protein 3; 3.6.3.44; ATP-binding cassette sub-family B member 4; P-glycoprotein 3; ABCB4; MDR3; PGY3; anti-ABCB4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Specificity: This ABCB4 antibody is generated from a mouse immunized with a recombinant protein of human ABCB4.
• Purity/Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 1279

• Applicable Applications for anti-ABCB4 antibody: ELISA, WB (Western Blot)
• Function: Energy-dependent phospholipid efflux translocator that acts as a positive regulator of biliary lipid secretion. Functions as a floppase that translocates specifically phosphatidylcholine (PC) from the inner to the outer leaflet of the canalicular membrane bilayer into the canaliculi of hepatocytes. Translocation of PC makes the biliary phospholipids available for extraction into the canaliculi lumen by bile salt mixed micelles and therefore protects the biliary tree from the detergent activity of bile salts (PubMed:7957936, PubMed:8898203, PubMed:9366571, PubMed:17523162, PubMed:23468132, PubMed:24806754, PubMed:24723470, PubMed:24594635, PubMed:21820390). Plays a role in the recruitment of phosphatidylcholine (PC), phosphatidylethanolamine (PE) and sphingomyelin (SM) molecules to nonraft membranes and to further enrichment of SM and cholesterol in raft membranes in hepatocytes (PubMed:23468132). Required for proper phospholipid bile formation (By similarity). Indirectly involved in cholesterol efflux activity from hepatocytes into the canalicular lumen in the presence of bile salts in an ATP-dependent manner (PubMed:24045840). Promotes biliary phospholipid secretion as canaliculi-containing vesicles from the canalicular plasma membrane (PubMed:9366571, PubMed:28012258). In cooperation with ATP8B1, functions to protect hepatocytes from the deleterious detergent activity of bile salts (PubMed:21820390). Does not confer multidrug resistance (By similarity).
• Cellular Location: Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}. Apical cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441}. Membrane raft. Cytoplasm Cytoplasmic vesicle, clathrin-coated vesicle {ECO:0000250|UniProtKB:Q08201}. Note=Localized at the apical canalicular membrane of the epithelial cells lining the lumen of the bile canaliculi and biliary ductules (By similarity). Transported from the Golgi to the apical bile canalicular membrane in a RACK1-dependent manner (PubMed:19674157). Redistributed into pseudocanaliculi formed between cells in a bezafibrate- or PPARA-dependent manner (PubMed:15258199). Localized preferentially in lipid nonraft domains of canalicular plasma membranes (PubMed:23468132) {ECO:0000250|UniProtKB:P21440, ECO:0000269|PubMed:15258199, ECO:0000269|PubMed:19674157, ECO:0000269|PubMed:23468132}
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Anti-ABCB4 Antibody at 1:4000 dilution + MCF-7 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 142 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-ABCB4 antibody

Crown Antibodies; Primary Antibodies; Metabolism; Signal Transduction

References

van der Bliek A.M.,et al.Gene 71:401-411 (1988). Hillier L.W.,et al.Nature 424:157-164 (2003). Scherer S.W.,et al.Science 300:767-772 (2003). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases. Smit J.J.,et al.Biochim. Biophys. Acta 1261:44-56 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 4505771
• NCBI GeneID: 5244
• NCBI Accession #: NP_000434.1
• NCBI GenBank Nucleotide #: NM_000443.3
• UniProt Accession #: P21439; Q14813; A0A2V7; A4D1D3; A4D1D4; A4D1D5; D6W5P3; D6W5P4
• Molecular Weight: Calculated MW: 141523
• NCBI Official Full Name: phosphatidylcholine translocator ABCB4 isoform A
• NCBI Official Synonym Full Names: ATP binding cassette subfamily B member 4
• NCBI Official Symbol: ABCB4
• NCBI Official Synonym Symbols: GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• NCBI Protein Information: phosphatidylcholine translocator ABCB4
• UniProt Protein Name: Phosphatidylcholine translocator ABCB4
• UniProt Gene Name: ABCB4