Mouse anti-Human, mouse ABL2 Monoclonal Antibody | anti-ABL2 antibody

ABL2 Antibody

Cat. Num. AAA283520

• Gene Names: ABL2; ARG; ABLL
• Reactivity: Human, mouse
• Applications: ELISA, Western Blot
• Purity: Purified Mouse Monoclonal Antibody (Mab)
• Synonyms: ABL2, Antibody; ABL2 Antibody; Abelson tyrosine-protein kinase 2; Abelson murine leukemia viral oncogene homolog 2; Abelson-related gene protein; Tyrosine-protein kinase ARG; ABL2; ABLL; ARG; anti-ABL2 antibody

• Host: Mouse
• Reactivity: Human, mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1442CT175.30.33
• Specificity: This ABL2 antibody is generated from a mouse immunized with a recombinant protein.
• Purity/Purification: Purified Mouse Monoclonal Antibody (Mab)
• Form/Format: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Length: 1064

• Applicable Applications for anti-ABL2 antibody: ELISA, WB (Western Blot)
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-ABL2 Antibody at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 128 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(Anti-ABL2 Antibody at 1:500 dilution + NIH/3T3 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 128 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-ABL2 antibody

Non-receptor tyrosine-protein kinase that plays an ABL1- overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement); CTTN (involved in signaling); or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin- bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1.

References

Kruh G.D.,et al.Proc. Natl. Acad. Sci. U.S.A. 87:5802-5806(1990).
Bianchi C.,et al.J. Cell. Biochem. 105:1219-1227(2008).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Gregory S.G.,et al.Nature 441:315-321(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 209862772
• NCBI GeneID: 27
• NCBI Accession #: NP_001129472.1
• NCBI GenBank Nucleotide #: NM_001136000.2
• UniProt Accession #: P42684; Q5T0X6; Q5W0C5; Q6NZY6; Q7Z301; A0M8X0; B7UEF2; B7UEF3; B7UEF4; B7UEF5
• Molecular Weight: 128343
• NCBI Official Full Name: Abelson tyrosine-protein kinase 2 isoform d
• NCBI Official Synonym Full Names: ABL proto-oncogene 2, non-receptor tyrosine kinase
• NCBI Official Symbol: ABL2
• NCBI Official Synonym Symbols: ARG; ABLL
• NCBI Protein Information: Abelson tyrosine-protein kinase 2
• UniProt Protein Name: Abelson tyrosine-protein kinase 2
• UniProt Gene Name: ABL2
• UniProt Synonym Gene Names: ABLL; ARG
• UniProt Entry Name: ABL2_HUMAN