WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Acetyl-p53- K370 Rabbit mAb (AAA283346) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
Rabbit anti-Human Acetyl-p53- K370 Monoclonal Antibody | anti-TP53 antibody
Acetyl-p53- K370 Rabbit mAb
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purification
Synonyms
Acetyl-p53- K370, Antibody; Acetyl-p53- K370 Rabbit mAb; P53; BCC7; LFS1; BMFS5; TRP53; Acetyl-p53- K370; anti-TP53 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Sequence
GGSRAHSSHLKSKKGQSTSRH
Applicable Applications for anti-TP53 antibody
ELISA, WB (Western Blot)
Cross Reactivity
Human, Mouse, Rat
Immunogen
A synthetic acetylated peptide around K370 of human p53 (P04637).
Modification
Acetyl K370
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Acetyl-p53- K370 Rabbit mAb (AAA283346) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of lysates from C6 cells, using Acetyl-p53- K370 Rabbit mAb (AAA283346) at 1:1000 dilution. C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)
WB (Western Blot)
(Western blot analysis of various lysates using Acetyl-p53- K370 Rabbit mAb (AAA283346) at 1:1000 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of various lysates using Acetyl-p53- K370 Rabbit mAb (AAA283346) at 1:1000 dilution. HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1s.)
Related Product Information for anti-TP53 antibody
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).
Product Categories/Family for anti-TP53 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated MW: 44kDa
Observed MW: 53kDa
Observed MW: 53kDa
UniProt Protein Name
Cellular tumor antigen p53
UniProt Gene Name
TP53
UniProt Synonym Gene Names
P53
UniProt Entry Name
P53_HUMAN
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Product Notes
The TP53 tp53 (Catalog #AAA283346) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Acetyl-p53- K370 Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Acetyl-p53- K370 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the TP53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GGSRAHSSHL KSKKGQSTSR H. It is sometimes possible for the material contained within the vial of "Acetyl-p53- K370, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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