IF (Immunofluorescence)
(Figure 1. Indirect immunofluorescence staining of frozen section of chicken gizzard showing specific positive staining of smooth muscle cells.)
Mouse actin alpha-muscle Monoclonal Antibody
Mouse anti actin alpha-muscle
Reactivity
Chicken, Human, Monkey, Rabbit, Rat, Swine, Zebrafish
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunoblot, Western Blot, ELISA
Synonyms
actin alpha-muscle, Antibody; Mouse anti actin alpha-muscle; anti-actin alpha-muscle antibody
Host
Mouse
Reactivity
Chicken, Human, Monkey, Rabbit, Rat, Swine, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
HHF35
Specificity
HHF35 reacts with both alpha-muscle and gamma-smooth muscle actin, and therefore reacts with skeletal muscle, cardiac muscle, vascular and visceral smooth muscle cells, pericytes and myoepithelial cells. It is also reactive in myofibroblasts. It does not react with epithelial, endothelial, neural or normal connective tissue cells when applied under the proper conditions to these tissue sections.
Form/Format
Each vial contains 50 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-actin alpha-muscle antibody
WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IB (Immunoblot), ELISA, EM (Immunoelectron Microscopy)
Source Note
HHF35 is a Mouse monoclonal IgG1 antibody derived by fusion of NS-1 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with an SDS-extracted protein fraction from Human myocardium.
Cross Reactivity
The epitope recognized by alpha-SM1 is highly conserved. The antibody therefore cross-reacts with many species including protochordates, lower craniates and mammals.
Product Type
Primary Antibodies
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
IF (Immunofluorescence)
(Figure 1. Indirect immunofluorescence staining of frozen section of chicken gizzard showing specific positive staining of smooth muscle cells.)
IF (Immunofluorescence)
(Figure 1. Indirect immunofluorescence staining of frozen section of chicken gizzard showing specific positive staining of smooth muscle cells.)
Related Product Information for anti-actin alpha-muscle antibody
Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (alpha-skeletal) and one in cardiac (alpha-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebRates. Alpha- muscle actin is present in striated as well as smooth muscle cells, and in pathological tissues derived therefrom. It has for example been detected in several types of muscle derived tumors, and also been shown to appear in stress fibers of fibroblastic cells involved in contractile phenomena such as wound healing and fibrocontractive diseases.
Product Categories/Family for anti-actin alpha-muscle antibody
References
Tsukada, T., Tippens, D., Gordon, D., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. American Journal of Pathology 126, 51-60.Tsukada, T., McNutt, M.A., Ross, R. and Gown, A.M. (1987). HHF35, a muscle-actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic Human tissues. American Journal of Pathology 127, 389-402.Schmidt, R.A., Cone, R., Haas, J.E. and Gown, A.M. (1988). Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed anti muscle actins. American Journal of Pathology 131, 19-28.Babaev, V.R., Bobryshev, Y.V., Stenina, O.V., Tararak, E.M. and Gabani, G. (1990). Heterogeneity of smooth muscle in atheromatous plaque of Human aorta. American Journal of Pathlogy 136, 1031-42.Nascimento, C., Caroli-Bottino, A., Paschoal, J. and Pannain, V.L. (2009). Vascular immunohistochemical markers: contributions to hepatocellular nodule diagnosis in explanted livers. Transplantation Proceedings 41, 4211-13.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
alpha-muscle actin
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Product Notes
The actin alpha-muscle (Catalog #AAA77499) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti actin alpha-muscle reacts with Chicken, Human, Monkey, Rabbit, Rat, Swine, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's actin alpha-muscle can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IB (Immunoblot), ELISA, EM (Immunoelectron Microscopy). Researchers should empirically determine the suitability of the actin alpha-muscle for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "actin alpha-muscle, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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