WB (Western Blot)
(Western Blot analysis of ACTb in various tissues of different speciesLane1: Rat brain, whole tissue lysateLane2: Rabbit, brainstem, whole tissue lysateLane3: Bovine kidney, whole tissue lysateLane4: Porcine liver, whole tissue lysateLane5: Caprine cerebellum, whole tissue lysateLane6: Canine spinal cord, whole tissue lysateLysates/proteins at 20ug per lane.Primary Ab: anti-ACTb antibody (AAA148990) at 0.3ng/mlSecondary Ab: HRP-conjugated Rabbit anti-mouse antibody at 1/10000 dilutionDeveloped using the ECL techniqueCalculated MW: 42KDObserved MW: 42KDExposure time: 1 min)
Mouse Actin Beta (ACTb) Monoclonal Antibody | anti-ACTb antibody
Anti-Actin Beta (ACTb) Monoclonal Antibody
Reactivity
Human, Mouse, Rat, Guinea pig, Rabbit, Dog, Pig, Bovine, Sheep, Horse, Chicken
Applications
Western Blot
Purity
Protein A+G Affinity Chromatography
Synonyms
Actin Beta (ACTb), Antibody; Anti-Actin Beta (ACTb) Monoclonal Antibody; anti-ACTb antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Guinea pig, Rabbit, Dog, Pig, Bovine, Sheep, Horse, Chicken
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C4
Purity/Purification
Protein A+G Affinity Chromatography
Form/Format
Liquid; Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-ACTb antibody
WB (Western Blot)
Species reactivity
Human; Mouse; Rat; Cavia; Canine; Porcine; Bovine; Ovine; Equine; Gallus
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the antibody at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the antibody at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Western Blot analysis of ACTb in various tissues of different speciesLane1: Rat brain, whole tissue lysateLane2: Rabbit, brainstem, whole tissue lysateLane3: Bovine kidney, whole tissue lysateLane4: Porcine liver, whole tissue lysateLane5: Caprine cerebellum, whole tissue lysateLane6: Canine spinal cord, whole tissue lysateLysates/proteins at 20ug per lane.Primary Ab: anti-ACTb antibody (AAA148990) at 0.3ng/mlSecondary Ab: HRP-conjugated Rabbit anti-mouse antibody at 1/10000 dilutionDeveloped using the ECL techniqueCalculated MW: 42KDObserved MW: 42KDExposure time: 1 min)
WB (Western Blot)
(Western Blot analysis of ACTb in various tissues of different speciesLane1: Rat brain, whole tissue lysateLane2: Rabbit, brainstem, whole tissue lysateLane3: Bovine kidney, whole tissue lysateLane4: Porcine liver, whole tissue lysateLane5: Caprine cerebellum, whole tissue lysateLane6: Canine spinal cord, whole tissue lysateLysates/proteins at 20ug per lane.Primary Ab: anti-ACTb antibody (AAA148990) at 0.3ng/mlSecondary Ab: HRP-conjugated Rabbit anti-mouse antibody at 1/10000 dilutionDeveloped using the ECL techniqueCalculated MW: 42KDObserved MW: 42KDExposure time: 1 min)
WB (Western Blot)
(Western Blot analysis of ACTb in various cell linesLane1: DU145 whole cell lysateLane2: HL60 whole cell lysateLane3: THP-1 whole cell lysateLane4: OS-RC-2 whole cell lysateLane5: MDA-MB-435S whole cell lysateLane6: T-47D whole cell lysateLysates/proteins at 20ug per lane.Primary Ab: anti-ACTb antibody (AAA148990) at 0.1ng/mlSecondary Ab: HRP-conjugated Rabbit anti-mouse antibody at 1/10000 dilutionDeveloped using the ECL technique.Calculated MW: 42KDObserved MW: 42KDExposure time: 1 min)
WB (Western Blot)
(Western Blot analysis of ACTb in various cell linesLane1: DU145 whole cell lysateLane2: HL60 whole cell lysateLane3: THP-1 whole cell lysateLane4: OS-RC-2 whole cell lysateLane5: MDA-MB-435S whole cell lysateLane6: T-47D whole cell lysateLysates/proteins at 20ug per lane.Primary Ab: anti-ACTb antibody (AAA148990) at 0.1ng/mlSecondary Ab: HRP-conjugated Rabbit anti-mouse antibody at 1/10000 dilutionDeveloped using the ECL technique.Calculated MW: 42KDObserved MW: 42KDExposure time: 1 min)
WB (Western Blot)
(Figure. Western Blot: Sample: Recombinant ACTb, Human)
WB (Western Blot)
(Figure. Western Blot: Sample: Recombinant ACTb, Human)
Product Categories/Family for anti-ACTb antibody
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Product Notes
The ACTb (Catalog #AAA148990) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Actin Beta (ACTb) Monoclonal Antibody reacts with Human, Mouse, Rat, Guinea pig, Rabbit, Dog, Pig, Bovine, Sheep, Horse, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Beta (ACTb) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ACTb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Beta (ACTb), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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