IF (Immunofluorescence)
(ICC/IF analysis of Adiponectin in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Adiponectin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human Adiponectin Monoclonal Antibody | anti-ADIPOQ antibody
Adiponectin antibody
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-G affinity chromatography
Synonyms
Adiponectin, Antibody; Adiponectin antibody; ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin globular domain; Mouse; Adipocyte; C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; Adipose most abunda; Adiponectin; 30 kDa adipocyte complement related protein; ACRP 30; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipose most abundant gene transcript 1; APM 1; GBP 28; Gelatin binding protein,; anti-ADIPOQ antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
5H7
Specificity
Can be stored at 2-8°C for 1 week. For long term storage, alqiuot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
244
Applicable Applications for anti-ADIPOQ antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen
Recombinant human adiponectin (15-244aa) purified from E. coli
Clone Note
Anti-human adiponectin mAb, clone 5H7, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Acrp30 protein.
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, alqiuot and store at -20°C to -80°C . Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of Adiponectin in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Adiponectin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of Adiponectin in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Adiponectin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate)
WB (Western Blot)
(The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate)
Related Product Information for anti-ADIPOQ antibody
Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
Product Categories/Family for anti-ADIPOQ antibody
References
Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-9.; Berg AH. et al., (2001) Nat Med; 7:947-53; Berg AH. et al., (2002) Trends Endocrinol Metab. 13: 84-89.; Yamauchi T. et al., (2002) Nat Med. 8:1288-95
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,414 Da
NCBI Official Full Name
adiponectin
NCBI Official Synonym Full Names
adiponectin, C1Q and collagen domain containing
NCBI Official Symbol
ADIPOQ
NCBI Official Synonym Symbols
ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
NCBI Protein Information
adiponectin
UniProt Protein Name
Adiponectin
UniProt Gene Name
ADIPOQ
UniProt Synonym Gene Names
ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1
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Product Notes
The ADIPOQ adipoq (Catalog #AAA47987) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Adiponectin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Adiponectin can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ADIPOQ adipoq for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Adiponectin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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