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IF (Immunofluorescence)
(Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
Mouse anti-Human AFP alpha 1 Fetoprotein Monoclonal Antibody | anti-AFP alpha 1 antibody
AFP alpha 1 Fetoprotein Monoclonal Antibody
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Synonyms
AFP alpha 1 Fetoprotein, Antibody; AFP alpha 1 Fetoprotein Monoclonal Antibody; AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; anti-AFP alpha 1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
The antibody detects endogenous AFP proteins.
Form/Format
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Applicable Applications for anti-AFP alpha 1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
Synthetic Peptide of AFP alpha 1 Fetoprotein
Preparation and Storage
Store at -20°C 1 year
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of Human-breast-cancer tissue. 1,AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue.1, AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200(4°C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue.1, AFP alpha 1 Fetoprotein Monoclonal Antibody(17C5) was diluted at 1:200(4°C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min).3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human gallbladder.1, Antibody was diluted at 1:100(4° overnight).2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval.3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded Human gallbladder.1, Antibody was diluted at 1:100(4° overnight).2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval.3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
WB (Western Blot)
(Western blot analysis of HepG2, diluted at 1:2,000.)
WB (Western Blot)
(Western blot analysis of HepG2, diluted at 1:2,000.)
Related Product Information for anti-AFP alpha 1 antibody
This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]
NCBI and Uniprot Product Information
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Product Notes
The AFP alpha 1 (Catalog #AAA314727) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AFP alpha 1 Fetoprotein Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AFP alpha 1 Fetoprotein can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the AFP alpha 1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFP alpha 1 Fetoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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