Application Data
Mouse anti-Human AHR Monoclonal Antibody | anti-AHR antibody
AHR (Aryl Hydrocarbon Receptor, Ah Receptor, AhR, Class E Basic Helix-loop-helix Protein 76, bHLHe76, BHLHE76) (APC)
Application Data
Application Data
Application Data
Application Data
Application Data
(Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)
Application Data
(Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence)
(Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)
IF (Immunofluorescence)
(Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)
IP (Immunoprecipitation)
(Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)
IP (Immunoprecipitation)
(Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)
WB (Western Blot)
(Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot)
(Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot)
(Western Blot detection against immunogen (36.74kD).)
WB (Western Blot)
(Western Blot detection against immunogen (36.74kD).)
NCBI and Uniprot Product Information
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Product Notes
The AHR ahr (Catalog #AAA24424) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AHR (Aryl Hydrocarbon Receptor, Ah Receptor, AhR, Class E Basic Helix-loop-helix Protein 76, bHLHe76, BHLHE76) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AHR can be used in a range of immunoassay formats including, but not limited to, FLISA, IF (Immunofluorescence), IP (Immunoprecipitation), WB (Western Blot). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AHR ahr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What cell types or tissues express human AHR that this antibody can detect?
The Aryl Hydrocarbon Receptor (AHR) is widely expressed across tissues, including intestinal epithelium, immune cells (T cells, dendritic cells, macrophages), liver, lung, skin, and nervous tissue. This antibody detects endogenous human AHR protein across diverse cell types and tissues, enabling comprehensive assessment of AHR expression patterns.
Can this anti-AHR antibody be used for Western blot, flow cytometry, or immunofluorescence?
Yes, this anti-AHR antibody is validated for multiple applications: Western blotting (quantifying AHR protein levels ~110 kDa), flow cytometry (identifying AHR+ immune cell populations), and immunofluorescence (assessing subcellular AHR localization). These complementary techniques provide a comprehensive characterization of AHR expression and activation status.
How does AHR activation affect gene expression or immune responses?
AHR is a ligand-activated transcription factor that, upon activation, translocates to the nucleus, forms heterodimers with ARNT, and drives expression of xenobiotic metabolism genes (CYP1A1, CYP1B1) plus immunomodulatory genes (IL-22, IL-17). AHR activation profoundly shapes Th17 cell differentiation, barrier immunity, and tumor-associated immune suppression.
Does this antibody detect ligand-bound versus unbound AHR forms?
This antibody typically detects total AHR protein regardless of ligand-binding status. However, AHR conformation changes upon activation (ligand binding and nuclear translocation) may affect antibody accessibility. Some applications (immunofluorescence showing nuclear vs. cytoplasmic distribution) indirectly assess AHR activation state based on subcellular localization patterns.
Is AHR expression upregulated by environmental toxins or xenobiotics?
AHR expression itself is not dramatically upregulated by xenobiotics, but AHR target gene expression is strongly activated by ligand binding. Classical xenobiotics (TCDD/dioxin, PAHs) potently activate AHR signaling. Importantly, endogenous AHR ligands (dietary components, microbial metabolites like kynurenine) and emerging environmental chemicals also activate AHR-dependent responses.
Can this antibody be used to study AHR signaling in cancer or immune regulation?
Yes, this antibody enables comprehensive AHR signaling studies in both contexts. In cancer, AHR shows context-dependent roles (tumor-suppressive in lung cancer, tumor-promoting in other contexts). In immunity, AHR profoundly regulates T cell differentiation, barrier immunity, and tumor immune escape, making this antibody invaluable for dissecting AHR's complex immunological functions.
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