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WB (Western Blot)
(Detection of human AHR by western blot. Samples: Whole cell lysate (50 ug) from KM12, HeLa, Hep-G2, HEK293T, GaMG, RT-4, Malme-3M, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
AHR recombinant antibody
Rabbit anti-AHR Recombinant Monoclonal Antibody [BLR118H]
Gene Names
AHR; bHLHe76
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified
Synonyms
AHR, Recombinant Antibody; Rabbit anti-AHR Recombinant Monoclonal Antibody [BLR118H]; AH-receptor; class E basic helix-loop-helix protein 76; bHLHe76; AhR; ah receptor; aryl hydrocarbon receptor; aromatic hydrocarbon receptor; Aryl hydrocarbon receptor; AHR recombinant antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
BLR118H
Purity/Purification
Purified
Form/Format
Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
Concentration
100ug/ml (varies by lot)
Applicable Applications for AHR recombinant antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 798 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human AHR by western blot. Samples: Whole cell lysate (50 ug) from KM12, HeLa, Hep-G2, HEK293T, GaMG, RT-4, Malme-3M, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot)
(Detection of human AHR by western blot. Samples: Whole cell lysate (50 ug) from KM12, HeLa, Hep-G2, HEK293T, GaMG, RT-4, Malme-3M, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IP (Immunoprecipitation)
(Detection of human AHR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1) used for IP at 20 ul/mg lysate. AHR was also immunoprecipitated by rabbit anti-AHR antibody BL1617B-4H10. For blotting immunoprecipitated AHR, AAA213589 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human AHR by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1) used for IP at 20 ul/mg lysate. AHR was also immunoprecipitated by rabbit anti-AHR antibody BL1617B-4H10. For blotting immunoprecipitated AHR, AAA213589 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohiostchemistry)
(Detection of human AHR in FFPE bladder carcinoma by immunohistochemistry. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohiostchemistry)
(Detection of human AHR in FFPE bladder carcinoma by immunohistochemistry. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human AHR in FFPE GaMG cells by immunocytochemistry. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry)
(Detection of human AHR in FFPE GaMG cells by immunocytochemistry. Antibody: Rabbit anti-AHR recombinant monoclonal antibody (AAA213589 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Related Product Information for AHR recombinant antibody
AHR is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. AHR has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, AHR is sequestered in the cytoplasm; upon ligand binding, the protein moves to the nucleus and stimulates transcription of target genes. [taken from NCBI Entrez Gene (Gene ID: 196)].
Product Categories/Family for AHR recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
96,147 Da
NCBI Official Full Name
aryl hydrocarbon receptor
NCBI Official Synonym Full Names
aryl hydrocarbon receptor
NCBI Official Symbol
AHR
NCBI Official Synonym Symbols
bHLHe76
NCBI Protein Information
aryl hydrocarbon receptor; AH-receptor; ah receptor; aromatic hydrocarbon receptor; class E basic helix-loop-helix protein 76
UniProt Protein Name
Aryl hydrocarbon receptor
UniProt Gene Name
AHR
UniProt Synonym Gene Names
BHLHE76; Ah receptor; AhR; bHLHe76
UniProt Entry Name
AHR_HUMAN
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Product Notes
The AHR ahr (Catalog #AAA213589) is a Recombinant Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-AHR Recombinant Monoclonal Antibody [BLR118H] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AHR can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the AHR ahr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHR, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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