IF (Immunofluorescence)
(Immunofluorescence of AKT1/2/3(green) in Hela cells using AKT Rabbit mAb at dilution 1/50, and DAPI(blue))
AKT Monoclonal Antibody | anti-AKT antibody
AKT Rabbit mAb
Gene Names
AKT3; MPPH; PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Synonyms
AKT, Antibody; AKT Rabbit mAb; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma; anti-AKT antibody
Host
Recombinant Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
S08-8F1
Purity/Purification
Affinity Purified
Form/Format
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA
Concentration
0.3mg/ml (varies by lot)
Applicable Applications for anti-AKT antibody
IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthetic peptide of human AKT1/2/3
Conjugation
Unconjugated
Modification
Unmodification
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence of AKT1/2/3(green) in Hela cells using AKT Rabbit mAb at dilution 1/50, and DAPI(blue))
IF (Immunofluorescence)
(Immunofluorescence of AKT1/2/3(green) in Hela cells using AKT Rabbit mAb at dilution 1/50, and DAPI(blue))
WB (Western Blot)
(Western blot detection of AKT1/2/3 in LnCap cell lysates using AKT Rabbit mAb(1:500 diluted).Predicted band size:56kDa.Observed band size:56kDa.)
WB (Western Blot)
(Western blot detection of AKT1/2/3 in LnCap cell lysates using AKT Rabbit mAb(1:500 diluted).Predicted band size:56kDa.Observed band size:56kDa.)
Related Product Information for anti-AKT antibody
AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
Product Categories/Family for anti-AKT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
55,775 Da
NCBI Official Full Name
protein kinase B gamma
NCBI Official Synonym Full Names
v-akt murine thymoma viral oncogene homolog 3
NCBI Official Symbol
AKT3
NCBI Official Synonym Symbols
MPPH; PKBG; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
NCBI Protein Information
RAC-gamma serine/threonine-protein kinase; PKB gamma; RAC-gamma serine/threonine protein kinase; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
UniProt Protein Name
RAC-gamma serine/threonine-protein kinase
UniProt Gene Name
AKT3
UniProt Synonym Gene Names
PKBG; PKB gamma
UniProt Entry Name
AKT3_HUMAN
Similar Products
Product Notes
The AKT akt3 (Catalog #AAA314303) is an Antibody produced from Recombinant Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKT Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKT can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the AKT akt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.