Application Data
(Profile of anti-Human ALDH1A1 reactivity on A549 cells analyzed by flow cytometry.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
Mouse anti-Human ALDH1A1 Monoclonal Antibody | anti-ALDH1A1 antibody
Anti-ALDH1A1 Antibody (PE), Mouse Monoclonal
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
ALDH1A1, Antibody; Anti-ALDH1A1 Antibody (PE), Mouse Monoclonal; ALDH1A1/ALDH1 Antibody (PE), Mouse MAb; aldehyde dehydrogenase 1 family, member A1; Anti-ALDC Antibody; Anti-ALDH-E1 Antibody; Anti-ALDH1 Antibody; Anti-ALDH11 Antibody; Anti-HEL-9 Antibody; Anti-HEL-S-53e Antibody; Anti-HEL12 Antibody; Anti-PUMB1 Antibody; Anti-RALDH1 Antibody; anti-ALDH1A1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
03
Specificity
Human ALDH1A1/ALDH1
Purity/Purification
Protein A
Form/Format
PBS solution containing 0.5% BSA and 0.03% ProClin300
Concentration
5ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-ALDH1A1 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Recombinant Human ALDH1A1 / ALDH1 protein (Catalog# )
Reagents
PE-conjugated Mouse monoclonal antibody
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ALDH1A1 / ALDH1 (rh ALDH1A1 / ALDH1; Catalog# MBS8120805; NP_000680.2; Ser2-Ser501) and conjugated with PE under optimum conditions, the unreacted PE was remove.
Preparation and Storage
This antibody can be stored at 2°C-8°C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze
Application Data
(Profile of anti-Human ALDH1A1 reactivity on A549 cells analyzed by flow cytometry.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
Application Data
(Profile of anti-Human ALDH1A1 reactivity on A549 cells analyzed by flow cytometry.Flow cytometry was performed on a BD FC Calibur flow cytometry system.)
Related Product Information for anti-ALDH1A1 antibody
Aldehyde dehydrogenase 1 family, member A1 (ALDH1A1), also known as Aldehyde dehydrogenase 1 (ALDH1), or Retinaldehyde Dehydrogenase 1 (RALDH1), is an enzyme that is expressed at high levels in stem cells and that has been suggested to regulate stem cell function. The retinaldehyde dehydrogenase (RALDH) subfamily of ALDHs, composed of ALDH1A1, ALDH1A2, ALDH1A3, and ALDH8A1, regulate development by catalyzing retinoic acid biosynthesis. The ALDH1A1 protein belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. ALDH1A1 also belongs to the group of corneal crystallins that help maintain the transparency of the cornea. Increased ALDH1A1 activity has been found in the stem cell populations of leukemia and some solid tumors. In tumor specimens, increased ALDH1A1 immunopositivity was found not only in secretory type cancer epithelial cells but also in neuroendocrine tumor populations. ALDH1 has been identified as a reliable marker of breast cancer stem cells. ALDH1 expression in primary cancer is an independent prognostic factor in node-positive breast cancer patients. ALDH1A1 plays a key role in normal hematopoiesis, and as a TLX1 transcriptional target, ALDH1A1 may contribute to the ability of this homeoprotein to alter cell fate and induce tumor growth.
References
Li T, et al. (2010). ALDH1A1 is a marker for malignant prostate stem cells and predictor of prostate cancer patients' outcome. Lab Invest. 90(2): 234-44.Levi BP, et al. (2009) Aldehyde dehydrogenase 1a1 is dispensable for stem cell function in the mouse hematopoietic and nervous systems. Blood. 113(8): 1670-80.Rahman FB, et al. (2006) Uncompetitive inhibition of Xenopus laevis aldehyde dehydrogenase 1A1 by divalent cations. Zoolog Sci. 23(3): 239-44.Jester JV, et al. (1999). The cellular basis of corneal transparency: evidence for corneal crystallins. J Cell Sci. 112 (Pt 5): 613-22.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,862 Da
NCBI Official Full Name
retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family, member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
NCBI Protein Information
retinal dehydrogenase 1; ALDH class 1; ALHDII; RALDH 1; acetaldehyde dehydrogenase 1; aldehyde dehydrogenase 1, soluble; aldehyde dehydrogenase, liver cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm bindi
UniProt Protein Name
Retinal dehydrogenase 1
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
UniProt Entry Name
AL1A1_HUMAN
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Product Notes
The ALDH1A1 aldh1a1 (Catalog #AAA255411) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ALDH1A1 Antibody (PE), Mouse Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH1A1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH1A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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