IF (Immunofluorescence)
(ICC/IF analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human ALDH5A1 Monoclonal Antibody | anti-ALDH5A1 antibody
ALDH5A1 antibody
Gene Names
ALDH5A1; SSDH; SSADH
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
ALDH5A1, Antibody; ALDH5A1 antibody; Succinate-semialdehyde dehydrogenase mitochondrial; SSADH; SSDH; Succinate-semialdehyde dehydrogenase; mitochondrial; anti-ALDH5A1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, k
Clone Number
AT17F5
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
535
Applicable Applications for anti-ALDH5A1 antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human ALDH5A1 (48-535aa) purified from E. coli
Clone Note
Anti-human ALDH5A1 mAb, clone AT17F5, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ALDH5A1 protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of ALDH5A1 in 293T cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDH5A1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The mouse liver tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot)
(The HepG2 cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The mouse liver tissue extract (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDH5A1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-ALDH5A1 antibody
This protein belongs to the aldehyde dehydrogenase family of proteins. This gene encodes a mitochondrial NAD+-dependent succinic semialdehyde dehydrogenase. A deficiency of this enzyme, known as 4-hydroxybutyricaciduria, is a rare inborn error in the metabolism of the neurotransmitter gamma-aminobutyric acid (GABA). In response to the defect, physiologic fluids from patients accumulate GHB, a compound with numerous neuromodulatory properties. Two transcript variants encoding distinct isoforms have been identified for this gene.
Product Categories/Family for anti-ALDH5A1 antibody
References
Chambliss KL., et al. (1995) The Journal of Biological Chemistry. 270 (1): 461-7.; Trettel F., et al. (1997) Advances in Experimental Medicine and Biology. 414: 253-60.; Kim YG., et al. (2009) EMBO J. 8;28 (7): 959-68.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,653 Da
NCBI Official Full Name
succinate-semialdehyde dehydrogenase, mitochondrial isoform 2
NCBI Official Synonym Full Names
aldehyde dehydrogenase 5 family member A1
NCBI Official Symbol
ALDH5A1
NCBI Official Synonym Symbols
SSDH; SSADH
NCBI Protein Information
succinate-semialdehyde dehydrogenase, mitochondrial
UniProt Protein Name
Succinate-semialdehyde dehydrogenase, mitochondrial
UniProt Gene Name
ALDH5A1
UniProt Synonym Gene Names
SSADH
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Product Notes
The ALDH5A1 aldh5a1 (Catalog #AAA48209) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALDH5A1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH5A1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ALDH5A1 aldh5a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH5A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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