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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Mouse anti-Human ALDH6A1 Monoclonal Antibody | anti-ALDH6A1 antibody
ALDH6A1 Antibody
Gene Names
ALDH6A1; MMSDH; MMSADHA
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Immunofluorescence
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
ALDH6A1, Antibody; ALDH6A1 Antibody; Methylmalonate-semialdehyde dehydrogenase [acylating]; mitochondrial; MMSDH; Malonate-semialdehyde dehydrogenase [acylating]; Aldehyde dehydrogenase family 6 member A1; ALDH6A1; anti-ALDH6A1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,IgK
Clone Number
147CT8.3.4
Specificity
This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
535
Applicable Applications for anti-ALDH6A1 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence)
Antigen Type
Recombinant Protein
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
WB (Western Blot)
(Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. AAA283519 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. AAA283519 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IF (Immunofluorescence)
(Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
IF (Immunofluorescence)
(Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody. AAA283519 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Related Product Information for anti-ALDH6A1 antibody
This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
Product Categories/Family for anti-ALDH6A1 antibody
References
Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Rose JE, et al. Mol Med, 2010 Jul-Aug. PMID 20379614.
Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Saito A, et al. J Hum Genet, 2009 Jun. PMID 19343046.
Physical mapping of CHX10, ALDH6A1, and ABCD4 on bovine chromosome 10q34. Kuiper H, et al. Cytogenet Genome Res, 2005. PMID 15909363.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
The human plasma proteome: a nonredundant list developed by combination of four separate sources. Anderson NL, et al. Mol Cell Proteomics, 2004 Apr. PMID 14718574.
Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. Saito A, et al. J Hum Genet, 2009 Jun. PMID 19343046.
Physical mapping of CHX10, ALDH6A1, and ABCD4 on bovine chromosome 10q34. Kuiper H, et al. Cytogenet Genome Res, 2005. PMID 15909363.
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). Gerhard DS, et al. Genome Res, 2004 Oct. PMID 15489334.
The human plasma proteome: a nonredundant list developed by combination of four separate sources. Anderson NL, et al. Mol Cell Proteomics, 2004 Apr. PMID 14718574.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
57840
NCBI Official Full Name
methylmalonate-semialdehyde dehydrogenase
NCBI Official Synonym Full Names
aldehyde dehydrogenase 6 family, member A1
NCBI Official Symbol
ALDH6A1
NCBI Official Synonym Symbols
MMSDH; MMSADHA
NCBI Protein Information
methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
UniProt Protein Name
Methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial
UniProt Gene Name
ALDH6A1
UniProt Synonym Gene Names
MMSDH; MMSDH; Malonate-semialdehyde dehydrogenase [acylating]
UniProt Entry Name
MMSA_HUMAN
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Product Notes
The ALDH6A1 aldh6a1 (Catalog #AAA283519) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALDH6A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH6A1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA, IF (Immunofluorescence). Researchers should empirically determine the suitability of the ALDH6A1 aldh6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH6A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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