IF (Immunofluorescence)
(ICC/IF analysis of ALDOC in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ALDOC in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Mouse anti-Human ALDOC Monoclonal Antibody | anti-ALDOC antibody
ALDOC antibody
Gene Names
ALDOC; ALDC
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Western Blot, ELISA
Purity
By protein-A affinity chromatography
Synonyms
ALDOC, Antibody; ALDOC antibody; Aldolase C fructose-bisphosphate.; Aldolase C; fructose-bisphosphate.; ALDC; anti-ALDOC antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
AT2E11
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
364
Applicable Applications for anti-ALDOC antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA
Antigen Species
Human
Immunogen
Recombinant human ALDOC (1-364aa) purified from E. coli
Clone Note
Anti-human ALDOC mAb, clone AT2E11, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ALDOC protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
IF (Immunofluorescence)
(ICC/IF analysis of ALDOC in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ALDOC in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
IF (Immunofluorescence)
(ICC/IF analysis of ALDOC in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). ICC/IF analysis of ALDOC in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ALDOC antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot)
(The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : PC3 cell lysate Lane 2. : U87MG cell lysate Lane 3. : WiDr cell lysate Lane 4. : Jurkat cell lysate Lane 5. : Mouse brain tissue lysate)
WB (Western Blot)
(The cell line and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ALDOC antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : PC3 cell lysate Lane 2. : U87MG cell lysate Lane 3. : WiDr cell lysate Lane 4. : Jurkat cell lysate Lane 5. : Mouse brain tissue lysate)
Related Product Information for anti-ALDOC antibody
ALDOC, also known as aldolase C, is a member of the class I fructose-biphosphate aldolase family. This protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehydes respectively. It is expressed specifically in the hippocampus and Purkinje cells of the brain.
Product Categories/Family for anti-ALDOC antibody
References
Wang CF., et al. (2007) Anim Genet. 38 (3): 203-210.; Arakaki TL., et al. (2004) Protein Sci. 13 (12): 3077-3084.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,456 Da
NCBI Official Full Name
fructose-bisphosphate aldolase C
NCBI Official Synonym Full Names
aldolase, fructose-bisphosphate C
NCBI Official Symbol
ALDOC
NCBI Official Synonym Symbols
ALDC
NCBI Protein Information
fructose-bisphosphate aldolase C
UniProt Protein Name
Fructose-bisphosphate aldolase C
UniProt Gene Name
ALDOC
UniProt Synonym Gene Names
ALDC
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Product Notes
The ALDOC aldoc (Catalog #AAA48208) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALDOC antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ALDOC can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ALDOC aldoc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDOC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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