WB (Western Blot)
(Anti-ALPL rabbit monoclonal antibody at 1:500 dilutionLane A: ALPL konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:95 kDa)
Rabbit anti-Human Alkaline Phosphatase/ALPL Monoclonal Antibody | anti-ALPL antibody
Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal
Gene Names
ALPL; HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
Reactivity
Human
Applications
Western Blot
Purity
Protein A
Synonyms
Alkaline Phosphatase/ALPL, Antibody; Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal; Alkaline Phosphatase/ALPL Antibody, Rabbit MAb; alkaline phosphatase, liver/bone/kidney; Anti-AP-TNAP Antibody; Anti-APTNAP Antibody; Anti-HOPS Antibody; Anti-TNAP Antibody; Anti-TNSALP Antibody; anti-ALPL antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
077
Specificity
Human Alkaline Phosphatase/ALPL
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-ALPL antibody
WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
A synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase/ALPL
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Alkaline Phosphatase/ALPL. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-ALPL rabbit monoclonal antibody at 1:500 dilutionLane A: ALPL konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:95 kDa)
WB (Western Blot)
(Anti-ALPL rabbit monoclonal antibody at 1:500 dilutionLane A: ALPL konckout Hela Whole Cell LysateLane B: Hela Whole Cell LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:95 kDa)
WB (Western Blot)
(Anti-ALPL rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:95 kDa)
WB (Western Blot)
(Anti-ALPL rabbit monoclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:95 kDa)
Related Product Information for anti-ALPL antibody
Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.
Product Categories/Family for anti-ALPL antibody
References
Brun-Heath I, et al. (2011) Differential expression of the bone and the liver tissue non-specific alkaline phosphatase isoforms in brain tissues. Cell Tissue Res. 343(3): 521-36.Whyte MP, et al. (1995) Alkaline phosphatase: placental and tissue-non-specific isoenzymes hydrolyze phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5-phosphate. J Clin Invest. 95: 1440-5.Whyte MP. (1994) Hypophosphatasia and the role of alkaline phosphatase in skeletal mineralization. Endocrinol Rev. 4: 439-61.Weinreb M, et al. (1990) Different pattern of alkaline phosphatase, osteopontin and osteocalcin expression in developing rat bone visualized by in situ hybridization. J Bone Miner Res. 5: 831-42.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
57kDa
NCBI Official Full Name
alkaline phosphatase, tissue-nonspecific isozyme isoform 1
NCBI Official Synonym Full Names
alkaline phosphatase, liver/bone/kidney
NCBI Official Symbol
ALPL
NCBI Official Synonym Symbols
HOPS; TNAP; APTNAP; TNSALP; AP-TNAP
NCBI Protein Information
alkaline phosphatase, tissue-nonspecific isozyme; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme
UniProt Protein Name
Alkaline phosphatase, tissue-nonspecific isozyme
UniProt Gene Name
ALPL
UniProt Synonym Gene Names
AP-TNAP; TNSALP
UniProt Entry Name
PPBT_HUMAN
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Product Notes
The ALPL alpl (Catalog #AAA254207) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Alkaline Phosphatase/ALPL Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alkaline Phosphatase/ALPL can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the ALPL alpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alkaline Phosphatase/ALPL, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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