WB (Western Blot)
(Anti-AFP rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa)
Rabbit anti-Human Alpha-fetoprotein Monoclonal Antibody | anti-AFP antibody
Recombinant Anti-Alpha-fetoprotein Antibody, Rabbit Monoclonal
Gene Names
AFP; FETA; HPAFP
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A
Synonyms
Alpha-fetoprotein, Antibody; Recombinant Anti-Alpha-fetoprotein Antibody, Rabbit Monoclonal; AFP/alpha-fetoprotein Antibody, Rabbit MAb; alpha-fetoprotein; Anti-AFPD Antibody; Anti-Alpha-fetoprotein Antibody; Anti-FETA Antibody; Anti-HPAFP Antibody; anti-AFP antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
003
Specificity
Human AFP/alpha-fetoprotein
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-AFP antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human AFP/alpha-fetoprotein protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human AFP/alpha-fetoprotein (rh AFP/alpha-fetoprotein).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Anti-AFP rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa)
WB (Western Blot)
(Anti-AFP rabbit monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:69 kDa)
IP (Immunoprecipitation)
(AFP was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-AFP rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-AFP rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size: 68 kDa)
IP (Immunoprecipitation)
(AFP was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-AFP rabbit monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-AFP rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 68 kDaObserved band size: 68 kDa)
Related Product Information for anti-AFP antibody
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
References
Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,678 Da
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The AFP afp (Catalog #AAA255533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Alpha-fetoprotein Antibody, Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Alpha-fetoprotein can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha-fetoprotein, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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