Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Mouse ALPHA FETOPROTEIN Monoclonal Antibody | anti-AFP antibody
MOUSE ANTI HUMAN ALPHA FETOPROTEIN
Gene Names
AFP; AFPD; FETA; HPAFP
Applications
ELISA
Synonyms
ALPHA FETOPROTEIN, Antibody; MOUSE ANTI HUMAN ALPHA FETOPROTEIN; anti-AFP antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
2I9/2
Specificity
This item recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults.
Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome.
This item does not cross-react with human albumin and other human serum proteins.
Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome.
This item does not cross-react with human albumin and other human serum proteins.
Form/Format
Purified
Ig fraction - liquid
Ig fraction - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
609
Applicable Applications for anti-AFP antibody
ELISA
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Ig fraction prepared by chromatography
Immunogen
Human fetal cord serum
Buffer Solution
Phosphate buffered saline
Target Species
Human
Preparation and Storage
Store at 4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (AAA49182) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein (AAA49182) as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Application Data
(Sandwich ELISA analysis of alpha fetoprotein binding using Mouse anti Human alpha fetoprotein as a capture reagent and biotinylated Mouse anti Human alpha fetoprotein as a detection reagent with human alpha fetoprotein as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Serum (green) and plasma (orange) samples are both displayed at 1:2 dilution.)
Related Product Information for anti-AFP antibody
MBS211649 recognises alpha fetoprotein (AFP), a member of the albuminoid superfamily. It is a 68kD plasma protein found in fetuses over four weeks old, produced by the yolk sac and the fetal liver, with the highest concentration reached during the 12th to 16th week of gestation. It binds and transports various ligands such as bilirubin, fatty acids, steroids, heavy metals and various drugs. After birth, plasma levels drop to trace amounts in healthy adults. Elevated AFP levels are indicative of certain cancers, particularly hepatocellular carcinoma, cancer of the testes or ovaries or a malignant teratoma. During pregnancy increased levels of AFP may indicate neural tube defects such as spina bifida, while decreased levels could indicate Down's syndrome. MBS211649 does not cross-react with human albumin and other human serum proteins.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
AFPD; FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein; alpha-1-fetoprotein; alpha-fetoglobulin
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The AFP afp (Catalog #AAA49182) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ALPHA FETOPROTEIN can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the AFP afp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALPHA FETOPROTEIN, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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