Mouse anti-Human AMP Deaminase, Isoform E (AMPD3) Monoclonal Antibody | anti-AMPD3 antibody

AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Mouse Monoclonal Antibody

Cat. Num. AAA62754

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
• Synonyms: AMP Deaminase, Isoform E (AMPD3), Antibody; AMP Deaminase, Isoform E (AMPD3) (Erythroid Marker) Mouse Monoclonal Antibody; Adenosine monophosphate deaminase (isoform E); AMP aminohydrolase; AMP deaminase 3; AMP deaminase isoform E; Ampd3; Erythrocyte AMP deaminase; Erythrocyte specific AMP deaminase; Erythrocyte specific protein; Erythrocyte type AMP deaminase; Myoadenylate deaminase; anti-AMPD3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: AMPD3/901
• Specificity: It recognizes a protein of ~90kDa, which is identified as Adenosine Monophosphate Deaminase, isoform E (AMPD3). It has 767 amino acids and is assigned an EC 3.5.4.6. It is a highly regulated enzyme that catalyzes the hydrolytic deamination of adenosine monophosphate to inosine monophosphate, a branch point in the adenylate catabolic pathway. AMPD3 gene encodes the erythrocyte (E) isoforms, whereas other family members encode isoforms that predominate in muscle (M) and liver (L) cells. This MAb shows reactivity with cells of the erythroid lineage at all stages of maturation in the peripheral blood, bone marrow, and fetal liver. Non-erythroid lineages are negative by flow cytometry. This MAb is useful in the erythroleukemia, identification of bone marrow erythroid precursors, gating erythroid nucleated precursor cells from malignant cells in bone marrow specimens.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 776

• Applicable Applications for anti-AMPD3 antibody: IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
• Cellular Localization: Cell Surface
• Immunogen: Recombinant full-length human AMDP3 protein
• Hu-Chromosome Location: 11p15.4
• Positive Control: RBC. Fetal liver; Spleen or Placenta
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

IHC (Immunohiostchemistry)

(Formalin-fixed, paraffin-embedded human Placenta stained with AMPD3 Monoclonal Antibody (AMPD3/901))

IHC (Immunohistochemistry)

(Formalin-fixed, paraffin-embedded human Tonsil stained with AMPD3 Monoclonal Antibody (AMPD3/901))

References

Sabina RL, Waldenstrm A, Ronquist G. The contribution of Ca+ calmodulin activation of human erythrocyte AMP deaminase (isoform E) to the erythrocyte metabolic dysregulation of familial phosphofructokinase deficiency. Haematologica. 2006;91(5):652-5

NCBI and Uniprot Product Information

• NCBI GI #: 4502079
• NCBI GeneID: 272
• NCBI Accession #: NP_000471.1
• NCBI GenBank Nucleotide #: NM_000480.2
• UniProt Accession #: Q01432; A0AUX0; B7Z2S2; B7Z763; B7Z877
• Molecular Weight: ~90kDa
• NCBI Official Full Name: AMP deaminase 3 isoform 1A
• NCBI Official Synonym Full Names: adenosine monophosphate deaminase 3
• NCBI Official Symbol: AMPD3
• NCBI Protein Information: AMP deaminase 3
• UniProt Protein Name: AMP deaminase 3
• UniProt Gene Name: AMPD3
• UniProt Entry Name: AMPD3_HUMAN