IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody)
Mouse ANAPC2 Monoclonal Antibody | anti-ANAPC2 antibody
ANAPC2 antibody
Gene Names
ANAPC2; APC2
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
ANAPC2 antibody was purified by affinity chromatography.
Synonyms
ANAPC2, Antibody; ANAPC2 antibody; Monoclonal ANAPC2; Anti-ANAPC2; Anaphase promoting complex subunit 2; APC2; KIAA1406; Cyclosome subunit 2; ANAPC2; ANAPC 2; ANAPC-2; anti-ANAPC2 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1A6
Purity/Purification
ANAPC2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
822
Applicable Applications for anti-ANAPC2 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry)
Biological Significance
A large protein complex, termed the anaphase-promoting complex (APC), or the cyclosome, promotes metaphase-anaphasetransition by ubiquitin ating its specific substrates such as mitotic cyclins and anaphase inhibitor, which aresubsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains eightsubunits. The composition of the APC is highly conserved in organisms from yeast to humans. ANAPC2 is a component of the complex and shares sequence similarity with a recently identified family of proteins calledcullins, which may also be involved in ubiquitin-mediated degradation.
Cross-Reactivity
Human, Rat, Monkey, Mouse
Immunogen
ANAPC2 antibody was raised in mouse using a full length recombinant protein of human ANAPC2 (NP_037498) produced in HEk293 cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody)
IF (Immunofluorescence)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ANAPC2 protein using ANAPC2 antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ANAPC2 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ANAPC2 antibody)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of ANAPC2 protein in paraffin embedded Adenocarcinoma of Human endometrium tissue using ANAPC2 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody)
WB (Western Blot)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ANAPC2 protein (Right) or empty vector (Left) detected with ANAPC2 antibody)
Related Product Information for anti-ANAPC2 antibody
Mouse monoclonal ANAPC2 antibody
Product Categories/Family for anti-ANAPC2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,469 Da
NCBI Official Full Name
anaphase-promoting complex subunit 2
NCBI Official Synonym Full Names
anaphase promoting complex subunit 2
NCBI Official Symbol
ANAPC2
NCBI Official Synonym Symbols
APC2
NCBI Protein Information
anaphase-promoting complex subunit 2
UniProt Protein Name
Anaphase-promoting complex subunit 2
UniProt Gene Name
ANAPC2
UniProt Synonym Gene Names
APC2; KIAA1406; APC2
UniProt Entry Name
ANC2_HUMAN
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Product Notes
The ANAPC2 anapc2 (Catalog #AAA106535) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ANAPC2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ANAPC2 anapc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANAPC2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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