WB (Western Blot)
(Western blot analysis of different cell lysates using mouse mAb to annexin A6, AAA76729, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.)
Mouse anti-Mouse Annexin A6 Monoclonal Antibody | anti-ANXA6 antibody
Annexin A6
Gene Names
ANXA6; ANX6; CBP68
Reactivity
Mouse
Applications
Immunofluorescence, Western Blot, Immunocytochemistry
Synonyms
Annexin A6, Antibody; Annexin A6; anti-ANXA6 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse IgG1
Form/Format
Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Applicable Applications for anti-ANXA6 antibody
IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant full length annexin A6 expressed in and purified from E coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different cell lysates using mouse mAb to annexin A6, AAA76729, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.)
WB (Western Blot)
(Western blot analysis of different cell lysates using mouse mAb to annexin A6, AAA76729, dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] dog A72, [7] equine NBL6 and [8] African green monkey COS1 cells. MCA-4G3 antibody detects annexin A6 protein with apparent MW of about 68kDa in all cell preparations. The blot was simultaneously probed with rabbit pAb to HSP60. The band at 60kDa corresponds to HSP60 protein.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, AAA76729, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A6, AAA76729, dilution 1:500 in red, and costained with chicken pAb to vimentin in green. The blue is Hoechst staining of nuclear DNA. The annexin A6 antibody detects protein predominantly expressed in spherical cellular membrane protrusions, known as blebs, while the vimentin antibody produces strong staining of theintermediate filament network of the cytoskeleton.)
Related Product Information for anti-ANXA6 antibody
The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner. Annexin A6 has a particularly surprising number of alternate names, 19 being listed on the Genecards ANXA6 site Annexin A6 is normally localized in the cytosol but becomes membrane associated following cellular injury, and so is often seen in ""blebs", transient herniations of the plasma membrane associated with apoptosis and more generally indicative of cell stress. Recent studies suggest that annexin A6 functions, in combination with annexin A4, in the repair of cell membranes following cellular injury.
This antibody was made against full length recombinant human annexin A6 expressed in and purified from E coli. The antibody recognizes annexin A6 in in human, rodents and many other mammals and can be used as a marker of blebs, indicative of cellular stress.
This antibody was made against full length recombinant human annexin A6 expressed in and purified from E coli. The antibody recognizes annexin A6 in in human, rodents and many other mammals and can be used as a marker of blebs, indicative of cellular stress.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,873 Da
NCBI Official Full Name
annexin A6 isoform 1
NCBI Official Synonym Full Names
annexin A6
NCBI Official Symbol
ANXA6
NCBI Official Synonym Symbols
ANX6; CBP68
NCBI Protein Information
annexin A6; p68; p70; CPB-II; annexin-6; calelectrin; lipocortin VI; calphobindin II; calphobindin-II; chromobindin-20; annexin VI (p68); 67 kDa calelectrin; calcium-binding protein p68
UniProt Protein Name
Annexin A6
UniProt Gene Name
ANXA6
UniProt Synonym Gene Names
ANX6; CPB-II
UniProt Entry Name
ANXA6_HUMAN
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Product Notes
The ANXA6 anxa6 (Catalog #AAA76729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Annexin A6 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A6 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the ANXA6 anxa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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