Application Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo)
Mouse annexin V Monoclonal Antibody | anti-ANXA5 antibody
Mouse anti annexin V
Gene Names
ANXA5; PP4; ANX5; ENX2
Reactivity
Human, Zebrafish
Applications
Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Synonyms
annexin V, Antibody; Mouse anti annexin V; anti-ANXA5 antibody
Host
Mouse
Reactivity
Human, Zebrafish
Clonality
Monoclonal
Isotype
IgG1
Clone Number
RUU-WAC2A
Specificity
RUU-WAC2A reacts anti Human recombinant and enDogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and Rat Annexin V in ELISA.
Form/Format
Each vial contains 100 ul of 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-ANXA5 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA
Source Note
RUU-WAC2a is a Mouse monoclonal IgG1 antibody obtained by fusion of P3-X63-Ag 8,653 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with recombinant annexin A5.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Application Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo)
Application Data
(Figure 1: Immunofluorescence staining of a 7 days old zebrafish embryo)
Related Product Information for anti-ANXA5 antibody
Annexin A5 (or annexin V) is a cellular protein of the annexin family. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin, and also to inhibit the activity of phospholipase A1. Annexin A5 forms a shield around negatively-charged phospholipid molecules, which blocks the entry of phospholipids into coagulation (clotting) reactions. Antibodies directed anti annexin A5 are the cause of the antiphospholipid syndrom in which the formation of the shield is disrupted by antibodies. Without the shield, there is an increased quantity of phospholipid molecules on cell membranes, speeding up coagulation reactions and causing the blood-clotting characteristic of the antiphospholipid antibody syndrome. Annexin A5 is used as a probe in the annexin A5 affinity assay to detect cells that have expressed phosphatidylserine on the cell surface, a feature found in apoptosis aswell as other forms of cell death (1,2). Platelets also expose phosphatidyl-serine on their surface when activated, which serves as binding site for various coagulation factors. Annexin A5 has been shown to interact with kinase insert domain receptor (3) and integrin beta 5 (4). RUU-WAC2a is a monoclonal that specifically reacts with annexin A5. This monoclonal antibody is directed to the phospholipid binding domain of annexin A5 and as such it inhibits annexin A5 binding to anionic phospholipid membranes for more than 95%. RUU-WAC 2A does not remove annexin A5 bound to phospholipid surfaces indiCating that the epitope is not accessible after interaction with annexin A5.
References
Koopman, G., Reutelingsperger, C.P., Kuijten, G.A.M. et al. (1994). Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-20. Vermes, I., Haanen, C., Steffens-Nakken, H. and Reutelingsperger, C. (1995). A novel assay for apoptosis-flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods 184, 39. Wen, Y., Edelman J.L., Kang, T. and Sachs, G. (1999). Lipocortin V may function as a signaling protein for vascular endothelial growth factor receptor-2/Flk-1. Biochem. Biophys. Res. Commun. 258, 713-21. Cardó-Vila, M., Arap, W. and Pasqualini, R. (2003). Alpha v beta 5 integrin-dependent programmed cell death triggered by a peptide mimic of annex V. Mol. Cell 31, 1151-62. Van Heerde, W.L. (1994). Thesis; localization, plasma levels and anticoagulant activity. Van Heerde, W.L., Reutelingsperger, C.P., Maassen, C., Lux, P., Derksen, R.H. and De Groot, P.G. (2003). The presence of antiphospholipid antibodies is not related to increased levels of annexin A5 in plasma. Jn Thromb Haemost. 3, 532-536. Van Heerde, W.L., Lap, P., Schoormans, S., De Groot, P.G., Reutelingsperger, C.P.M. and Vroom, T.M. (2004). Localization of annexin A5 in Human tissues. Annexins 1, 37-43
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,937 Da
NCBI Official Full Name
annexin A5
NCBI Official Synonym Full Names
annexin A5
NCBI Official Symbol
ANXA5
NCBI Official Synonym Symbols
PP4; ANX5; ENX2
NCBI Protein Information
annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; OTTHUMP00000164026; OTTHUMP00000219982; OTTHUMP00000219983; OTTHUMP00000219985; thromboplastin inhibitor; vascular anticoagulant-alpha; placental anticoagulant protein 4; placental anticoagulant protein I
UniProt Protein Name
Annexin A5
UniProt Gene Name
ANXA5
UniProt Synonym Gene Names
ANX5; ENX2; PP4
UniProt Entry Name
ANXA5_HUMAN
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Product Notes
The ANXA5 anxa5 (Catalog #AAA77497) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti annexin V reacts with Human, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's annexin V can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA. Researchers should empirically determine the suitability of the ANXA5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "annexin V, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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