FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279176 (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.)
ANXA4 recombinant antibody
ANXA4 Recombinant Monoclonal Antibody
Gene Names
ANXA4; ANX4; PIG28; ZAP36; HEL-S-274
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Affinity purified
Synonyms
ANXA4, Recombinant Antibody; ANXA4 Recombinant Monoclonal Antibody; 35 beta calcimedin; 35-beta calcimedin; AIV; Annexin 4; Annexin A4; Annexin IV (placental anticoagulant protein II); Annexin IV; Annexin IV placental anticoagulant protein II; Annexin-4; AnnexinA4; AnnexinIV; ANX 4; ANX A4; ANX4; ANXA 4; ANXA4; ANXA4 protein; ANXA4_HUMAN; Carbohydrate binding protein P33/P41; Carbohydrate-binding protein p33/p41; Chromobindin 4; Chromobindin-4; Chromobindin4; DKFZp686H02120; Endonexin I; EndonexinI; Lipocortin IV; LipocortinIV; MGC75105; OTTHUMP00000160052; OTTHUMP00000202207; P32.5; P33/41; PAP II; PAP-II; PAPII; PIG 28; PIG28; Placental anticoagulant protein II; PP4 X; PP4-X; PP4X; Proliferation inducing gene 28; Proliferation inducing protein 28; Protein II; Xanx-4; ZAP 36; ZAP36; ANXA4 recombinant antibody
Host
HEK293F Cell
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG
Clone Number
31D6
Purity/Purification
Affinity purified
Form/Format
Liquid; 0.03% Proclin 300, 50% glycerol, 0.01M PBS, pH 7.4.
Applicable Applications for ANXA4 recombinant antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Recombinant Human ANXA4 protein
Conjugation
Non-conjugated
Research Area
Cardiovascular; Signal transduction
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279176 (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.)
FCM/FACS (Flow Cytometry)
(Overlay Peak curve showing Hela cells stained with AAA279176 (red line) at 1:800. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1ug/1*106cells) used under the same conditions. Acquisition of >10,017 events was performed.)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue lysateAll lanes: ANXA4 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 kDaObserved band size: 36 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF7 whole cell lysate, U251 whole cell lysate, PC-3 whole cell lysate, Mouse Liver tissue lysateAll lanes: ANXA4 antibody at 1:500SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 kDaObserved band size: 36 kDa)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,063 Da
NCBI Official Full Name
annexin A4
NCBI Official Synonym Full Names
annexin A4
NCBI Official Symbol
ANXA4
NCBI Official Synonym Symbols
ANX4; PIG28; ZAP36; HEL-S-274
NCBI Protein Information
annexin A4; 35-beta calcimedin; P32.5; PAP-II; PP4-X; annexin IV (placental anticoagulant protein II); annexin-4; carbohydrate-binding protein p33/p41; chromobindin-4; endonexin I; epididymis secretory protein Li 274; lipocortin IV; proliferation-inducing
UniProt Protein Name
Annexin A4
UniProt Gene Name
ANXA4
UniProt Synonym Gene Names
ANX4; PAP-II
UniProt Entry Name
ANXA4_HUMAN
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Product Notes
The ANXA4 anxa4 (Catalog #AAA279176) is a Recombinant Antibody produced from HEK293F Cell and is intended for research purposes only. The product is available for immediate purchase. The ANXA4 Recombinant Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ANXA4 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the ANXA4 anxa4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANXA4, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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