Arginase 1 Recombinant Antibody | Arg1 recombinant antibody

Rabbit anti-Arginase 1 Recombinant Monoclonal Antibody [BLR161J]

Cat. Num. AAA213618

• Gene Names: Arg1; AI; PGIF; Arg-1; AI256583
• Reactivity: Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified
• Synonyms: Arginase 1, Recombinant Antibody; Rabbit anti-Arginase 1 Recombinant Monoclonal Antibody [BLR161J]; PGIF; type I arginase; liver-type arginase; arginase-1; arginase I; arginase 1, liver; Arg-1; AI256583; AI; Arginase-1; arginase, liver; Arg1 recombinant antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Clone Number: BLR161J
• Purity/Purification: Purified
• Form/Format: Liquid. Whole IgG. Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and 0.09% Sodium Azide
• Concentration: 100ug/ml (varies by lot)

• Applicable Applications for Arg1 recombinant antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Residues 1-322 (FL)
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of mouse Arginase 1 by western blot. Samples: Lysate from from H-4-II-E, NIH 3T3, mouse liver, TCMK-1, and AML12 cells. Antibody: Rabbit anti-Arginase-1 recombinant monoclonal antibody (AAA213618 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation)

(Detection of mouse Arginase 1 by western blot of immunoprecipitates. Samples: Tissue lysate (1.0 mg per IP reaction; 20% of IP loaded) from mouse liver cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Arginase 1 recombinant monoclonal antibody (AAA213618 lot 1) used at 20 ul/mg lysate. For blotting immunoprecipitated Arginase 1, AAA213618 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry)

(Detection of mouse Arginase 1 in FFPE mouse liver by immunohistochemistry. Antibody: Rabbit anti-Arginase 1 recombinant monoclonal antibody (AAA213618 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

Related Product Information for Arg1 recombinant antibody

Arginase1 is a key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively; the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia.  [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P05089].

Product Categories/Family for Arg1 recombinant antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 7106255
• NCBI GeneID: 11846
• NCBI Accession #: NP_031508.1
• NCBI GenBank Nucleotide #: NM_007482.3
• UniProt Accession #: Q61176; Q3TB74; Q3UEL0; Q4FK78; Q80VI4
• Molecular Weight: 34,808 Da
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase, liver
• NCBI Official Symbol: Arg1
• NCBI Official Synonym Symbols: AI; PGIF; Arg-1; AI256583
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• UniProt Gene Name: Arg1