WB (Western Blot)
(Western blot analysis of lysates from THP-1 cell line, mouse liver, mouse testis tissue (from left to right), using ATG3 Antibody. AAA283453 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
Mouse anti-Human, mouse ATG3 Monoclonal Antibody | anti-ATG3 antibody
ATG3 Antibody
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
ATG3, Antibody; ATG3 Antibody; Ubiquitin-like-conjugating enzyme ATG3; 632-; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3; APG3; APG3L; anti-ATG3 antibody
Host
Mouse
Reactivity
Human, mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
1377CT239.6.1.12
Specificity
This ATG3 antibody is generated from a mouse immunized with recombinant protein of human ATG3.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
311
Applicable Applications for anti-ATG3 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from THP-1 cell line, mouse liver, mouse testis tissue (from left to right), using ATG3 Antibody. AAA283453 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from THP-1 cell line, mouse liver, mouse testis tissue (from left to right), using ATG3 Antibody. AAA283453 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using ATG3 Antibody. AAA283453 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using ATG3 Antibody. AAA283453 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using ATG3 Antibody. AAA283453 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. kidney section using ATG3 Antibody. AAA283453 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Related Product Information for anti-ATG3 antibody
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
Product Categories/Family for anti-ATG3 antibody
References
Tanida I.,et al.J. Biol. Chem. 277:13739-13744(2002).
Wu B.X.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Tanida I.,et al.Biochem. Biophys. Res. Commun. 296:1164-1170(2002).
Wu B.X.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Tanida I.,et al.Biochem. Biophys. Res. Commun. 296:1164-1170(2002).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35864
NCBI Official Full Name
ubiquitin-like-conjugating enzyme ATG3 isoform 2
NCBI Official Synonym Full Names
autophagy related 3
NCBI Official Symbol
ATG3
NCBI Official Synonym Symbols
APG3; APG3L; PC3-96; APG3-LIKE
NCBI Protein Information
ubiquitin-like-conjugating enzyme ATG3
UniProt Protein Name
Ubiquitin-like-conjugating enzyme ATG3
UniProt Gene Name
ATG3
UniProt Synonym Gene Names
APG3; APG3L; APG3-like; hApg3
UniProt Entry Name
ATG3_HUMAN
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Product Notes
The ATG3 atg3 (Catalog #AAA283453) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATG3 Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the ATG3 atg3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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