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WB (Western Blot)
(Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (M09565).)
Mouse ATP5H Monoclonal Antibody | anti-ATP5H antibody
Anti-ATP5H Antibody (Monoclonal, 6B12)
Gene Names
ATP5PD; ATPQ; APT5H; ATP5H
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
ATP5H, Antibody; Anti-ATP5H Antibody (Monoclonal, 6B12); ATP synthase D chain mitochondrial antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit antibody; ATP synthase H+ transporting mitochondrial F1F0 subunit d antibody; ATP synthase subunit d antibody; ATP synthase subunit d, mitochondrial antibody; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit d antibody; ATP5H antibody; ATP5H_HUMAN antibody; ATP5JD antibody; ATPase subunit d antibody; ATPQ antibody; mitochondrial antibody; My032 protein antibody; anti-ATP5H antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6B12
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
137
Applicable Applications for anti-ATP5H antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
E Coli-derived human ATP5H recombinant protein (Position: A2-L161). Human ATP5H shares 81% and 78% amino acid (aa) sequence identity with mouse and rat ATP5H, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (M09565).)
WB (Western Blot)
(Figure 1. Western blot analysis of ATP5H using anti-ATP5H antibody (M09565).)
IHC (Immunohistochemisry)
IHC (Immunohistochemisry)
IHC (Immunohiostchemistry)
IHC (Immunohiostchemistry)
IF (Immunofluorescence)
IF (Immunofluorescence)
Related Product Information for anti-ATP5H antibody
Description: Mouse IgG monoclonal antibody for ATP5H detection. Tested with WB, IHC-P, ICC/IF in Human;Rat;Mouse;Monkey.
Background: ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
Background: ATP5H is also known as ATPQ. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15.
References
1. Boada M, et al. ATP5H/KCTD2 locus is associated with Alzheimer's disease risk. Mol Psychiatry, 2014 Jun.
2. Kim DW, et al. Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells. Cancer Sci, 2008 Oct.
2. Kim DW, et al. Identification of mitochondrial F(1)F(0)-ATP synthase interacting with galectin-3 in colon cancer cells. Cancer Sci, 2008 Oct.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
ATP synthase subunit d, mitochondrial isoform b
NCBI Official Synonym Full Names
ATP synthase peripheral stalk subunit d
NCBI Official Symbol
ATP5PD
NCBI Official Synonym Symbols
ATPQ; APT5H; ATP5H
NCBI Protein Information
ATP synthase subunit d, mitochondrial
UniProt Protein Name
ATP synthase subunit d, mitochondrial
UniProt Gene Name
ATP5H
UniProt Synonym Gene Names
ATPase subunit d
UniProt Entry Name
ATP5H_HUMAN
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Product Notes
The ATP5H atp5h (Catalog #AAA125507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-ATP5H Antibody (Monoclonal, 6B12) reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5H can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ATP5H atp5h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5H, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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