FCM/FACS (Flow Cytometry)
(Flow Cytometry / FACS analysis: Detection of ATPase Na+/K+ alpha 1 in fixed Hela cells)
Mouse ATPase Na+/K+ transporting alpha 1 Monoclonal Antibody | anti-ATP1A1 antibody
ATPase Na+/K+ transporting alpha 1, Monoclonal, Mouse
Reactivity
Cat, Human, Mouse, Primate, Rat, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay
Synonyms
ATPase Na+/K+ transporting alpha 1, Antibody; ATPase Na+/K+ transporting alpha 1, Monoclonal, Mouse; anti-ATP1A1 antibody
Host
Mouse
Reactivity
Cat, Human, Mouse, Primate, Rat, Rabbit
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
464.6
Form/Format
50ul, Ascites Fluid with 0.1% Sodium Azide as a preservative.
Sequence Length
1023
Applicable Applications for anti-ATP1A1 antibody
WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry)
Type Info
Mouse IgG
Immunogen
Purified Na+,K+-ATPase from rabbit renal outer medulla.
Preparation and Storage
Store frozen. Aliquot as undiluted antisera and immediately place at -20 degree C. Antisera may have become trapped in top of vial during shipping. Centrifugation of vial is recommended before opening. Stable for at least 6months at -20 degree C. Repeated freeze/thaw cycles compromise the integrity of the antiserum.
FCM/FACS (Flow Cytometry)
(Flow Cytometry / FACS analysis: Detection of ATPase Na+/K+ alpha 1 in fixed Hela cells)
FCM/FACS (Flow Cytometry)
(Flow Cytometry / FACS analysis: Detection of ATPase Na+/K+ alpha 1 in fixed Hela cells)
Application Data
(Image: Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using ATPase Na+/K+ alpha 1. Nuclei (Blue) were counterstained using Hoechst 33258.)
Application Data
(Image: Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using ATPase Na+/K+ alpha 1. Nuclei (Blue) were counterstained using Hoechst 33258.)
WB (Western Blot)
(Western Blotting: Detection of ATPase Na+/K+ alpha 1 in in porcine proximal tubule protein)
WB (Western Blot)
(Western Blotting: Detection of ATPase Na+/K+ alpha 1 in in porcine proximal tubule protein)
Related Product Information for anti-ATP1A1 antibody
is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, wherease the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. This ATPase Na+/K+ transporting alpha 1 is expressed in both neuronal and non-neuronal cells.
Product Categories/Family for anti-ATP1A1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
113,000 Da
NCBI Official Full Name
ATPase, Na+/K+ transporting, alpha 1 polypeptide, isoform CRA_e
NCBI Official Synonym Full Names
ATPase Na+/K+ transporting subunit alpha 1
NCBI Official Symbol
ATP1A1
NCBI Protein Information
sodium/potassium-transporting ATPase subunit alpha-1
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit alpha-1
UniProt Gene Name
ATP1A1
UniProt Synonym Gene Names
Na(+)/K(+) ATPase alpha-1 subunit
UniProt Entry Name
AT1A1_HUMAN
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Product Notes
The ATP1A1 atp1a1 (Catalog #AAA76410) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATPase Na+/K+ transporting alpha 1, Monoclonal, Mouse reacts with Cat, Human, Mouse, Primate, Rat, Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase Na+/K+ transporting alpha 1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the ATP1A1 atp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATPase Na+/K+ transporting alpha 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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