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IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Ovarian cancer Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
Mouse ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Monoclonal Antibody | anti-ATP1a1 antibody
Monoclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)
Reactivity
Human, Mouse, Rat, Rabbit, Pig, Bovine
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G Affinity Chromatography
Synonyms
ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1), Antibody; Monoclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1); Sodium pump subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium/potassium-transporting ATPase subunit alpha-1; anti-ATP1a1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit, Pig, Bovine
Clonality
Monoclonal
Clone Number
C206
Specificity
The antibody is a mouse monoclonal antibody raised against ATP1a1. It has been selected for its ability to recognize ATP1a1 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence
His493~Ala660
Applicable Applications for anti-ATP1a1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
IHC: 5-20ug/mL; 1:50-200
Immunogen Sequence
His493~Ala660
Cross Reactivity
Human
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Ovarian cancer Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P; Samples: Human Ovarian cancer Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human amygdalitis Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human amygdalitis Tissue; Primary Ab: 10?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Human ATP1a1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
IHC (Immunohistochemistry)
(DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20ug/ml Mouse Anti-Human ATP1a1 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Porcine Heart lysate; Lane3: Rat Heart lysate; Lane4: Bovine Heart lysate; Lane5: Rabbit Heart lysate; Lane6: Porcine Cerebrum lysatePrimary Ab: 2?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Porcine Heart lysate; Lane3: Rat Heart lysate; Lane4: Bovine Heart lysate; Lane5: Rabbit Heart lysate; Lane6: Porcine Cerebrum lysatePrimary Ab: 2?g/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Human A549 cell lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Human A549 cell lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot; Sample: Mouse Heart lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 Antibody Secon)
WB (Western Blot)
(Western Blot; Sample: Mouse Heart lysate; Primary Ab: 5ug/ml Mouse Anti-Human ATP1a1 Antibody Secon)
Product Categories/Family for anti-ATP1a1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,896 Da
NCBI Official Full Name
sodium/potassium-transporting ATPase subunit alpha-1 isoform a
NCBI Official Synonym Full Names
ATPase, Na+/K+ transporting, alpha 1 polypeptide
NCBI Official Symbol
ATP1A1
NCBI Protein Information
sodium/potassium-transporting ATPase subunit alpha-1; sodium pump 1; Na+/K+ ATPase 1; Na,K-ATPase alpha-1 subunit; sodium pump subunit alpha-1; Na+, K+ ATPase alpha subunit; Na(+)/K(+) ATPase alpha-1 subunit; Na, K-ATPase, alpha-A catalytic polypeptide; s
UniProt Protein Name
Sodium/potassium-transporting ATPase subunit alpha-1
UniProt Gene Name
ATP1A1
UniProt Synonym Gene Names
Na(+)/K(+) ATPase alpha-1 subunit
UniProt Entry Name
AT1A1_HUMAN
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Product Notes
The ATP1a1 atp1a1 (Catalog #AAA21160) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) reacts with Human, Mouse, Rat, Rabbit, Pig, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the ATP1a1 atp1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: His493~Ala 660. It is sometimes possible for the material contained within the vial of "ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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