WB (Western Blot)
(Western blot analysis of lysate from HT-29 cell line, using AURKA Antibody. AAA283448 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
Mouse anti-Human AURKA Monoclonal Antibody | anti-AURKA antibody
AURKA Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Mouse Monoclonal Antibody (Mab)
Synonyms
AURKA, Antibody; AURKA Antibody; Aurora kinase A; Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; AURKA; AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1; anti-AURKA antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Specificity
This AURKA antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between amino acids from the human region of human AURKA.
Purity/Purification
Purified Mouse Monoclonal Antibody (Mab)
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Sequence Length
403
Applicable Applications for anti-AURKA antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Cellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:P97477}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole {ECO:0000250|UniProtKB:P97477}. Cell projection, neuron projection {ECO:0000250|UniProtKB:P97477}. Note=Detected at the neurite hillock in developing neurons (By similarity). Localizes at the centrosome in mitotic cells from early prophase until telophase, but also localizes to the spindle pole MTs from prophase to anaphase (PubMed:9606188, PubMed:17229885, PubMed:21225229). Colocalized with SIRT2 at centrosome (PubMed:22014574). Moves to the midbody during both telophase and cytokinesis (PubMed:17726514). Associates with both the pericentriolar material (PCM) and centrioles (PubMed:22014574). The localization to the spindle poles is regulated by AAAS (PubMed:26246606) {ECO:0000250|UniProtKB:P97477, ECO:0000269|PubMed:17229885, ECO:0000269|PubMed:17726514, ECO:0000269|PubMed:21225229, ECO:0000269|PubMed:22014574, ECO:0000269|PubMed:26246606, ECO:0000269|PubMed:9606188}
Tissue Location
Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysate from HT-29 cell line, using AURKA Antibody. AAA283448 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
WB (Western Blot)
(Western blot analysis of lysate from HT-29 cell line, using AURKA Antibody. AAA283448 was diluted at 1:1000. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35ug.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using AURKA Antibody. AAA283448 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded H. brain section using AURKA Antibody. AAA283448 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)
Related Product Information for anti-AURKA antibody
Function:
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed: 26246606, PubMed: 12390251, PubMed: 18615013, PubMed: 11039908, PubMed: 17125279, PubMed: 17360485). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed: 26246606, PubMed: 14523000). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed: 27335426). Required for initial activation of CDK1 at centrosomes (PubMed: 13678582, PubMed: 15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed: 18056443, PubMed: 15128871, PubMed: 14702041, PubMed: 11551964, PubMed: 15147269, PubMed: 15987997, PubMed: 17604723, PubMed: 18615013). Regulates KIF2A tubulin depolymerase activity (PubMed: 19351716). Important for microtubule formation and/or stabilization (PubMed: 18056443). Required for normal axon formation (PubMed: 19812038). Plays a role in microtubule remodeling during neurite extension (PubMed: 19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint- response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed: 14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed: 11551964). Necessary for proper cilia disassembly prior to mitosis (PubMed: 17604723, PubMed: 20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed: 28218735).
Background:
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
Mitotic serine/threonine kinase that contributes to the regulation of cell cycle progression (PubMed: 26246606, PubMed: 12390251, PubMed: 18615013, PubMed: 11039908, PubMed: 17125279, PubMed: 17360485). Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis (PubMed: 26246606, PubMed: 14523000). Required for normal spindle positioning during mitosis and for the localization of NUMA1 and DCTN1 to the cell cortex during metaphase (PubMed: 27335426). Required for initial activation of CDK1 at centrosomes (PubMed: 13678582, PubMed: 15128871). Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2 (PubMed: 18056443, PubMed: 15128871, PubMed: 14702041, PubMed: 11551964, PubMed: 15147269, PubMed: 15987997, PubMed: 17604723, PubMed: 18615013). Regulates KIF2A tubulin depolymerase activity (PubMed: 19351716). Important for microtubule formation and/or stabilization (PubMed: 18056443). Required for normal axon formation (PubMed: 19812038). Plays a role in microtubule remodeling during neurite extension (PubMed: 19668197). Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint- response pathways critical for oncogenic transformation of cells, by phosphorylating and destabilizing p53/TP53 (PubMed: 14702041). Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity (PubMed: 11551964). Necessary for proper cilia disassembly prior to mitosis (PubMed: 17604723, PubMed: 20643351). Regulates protein levels of the anti-apoptosis protein BIRC5 by suppressing the expression of the SCF(FBXL7) E3 ubiquitin-protein ligase substrate adapter FBXL7 through the phosphorylation of the transcription factor FOXP1 (PubMed: 28218735).
Background:
Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.
Product Categories/Family for anti-AURKA antibody
References
Kimura M.,et al.J. Biol. Chem. 272:13766-13771(1997).
Shindo M.,et al.Biochem. Biophys. Res. Commun. 244:285-292(1998).
Zhou H.,et al.Nat. Genet. 20:189-193(1998).
Wang L.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 414:865-871(2001).
Shindo M.,et al.Biochem. Biophys. Res. Commun. 244:285-292(1998).
Zhou H.,et al.Nat. Genet. 20:189-193(1998).
Wang L.,et al.Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases.
Deloukas P.,et al.Nature 414:865-871(2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
45809
NCBI Official Full Name
aurora kinase A
NCBI Official Synonym Full Names
aurora kinase A
NCBI Official Symbol
AURKA
NCBI Official Synonym Symbols
AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47
NCBI Protein Information
aurora kinase A
UniProt Protein Name
Aurora kinase A
UniProt Gene Name
AURKA
UniProt Synonym Gene Names
AIK; AIRK1; ARK1; AURA; AYK1; BTAK; IAK1; STK15; STK6; ARK-1; Aurora-related kinase 1; hARK1
UniProt Entry Name
AURKA_HUMAN
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Product Notes
The AURKA aurka (Catalog #AAA283448) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AURKA Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AURKA can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the AURKA aurka for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AURKA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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